Recognition and function analysis of MKK6 in immune reaction against Vibrio Parahaemolyticus in Crassostrea hongkongensis
4 affiliations
- 1Fishery College of Guangdong Ocean University, Zhanjiang, China
- 2Academician Joint Laboratory of Germplasm Resource Exploitation, Utilization and Health Assessment for Aquatic Animal, Guangdong Ocean University, Zhanjiang, China
- 3Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fisheries, Guangdong Ocean University, Zhanjiang, China
- 4FangchenggangVocational and Technical College, Fangchenggang, China
Abstract
MKK6 is a key protein kinase that regulates inflammatory response under pathogen infection by activating MAPK signal pathway. However, the function of MKK6 remained unclear within oysters. Our research recognized a novel KK6 gene from C. hongkongensis, which is named ChMKK6. The cDNA of ChMKK6 gene was 2,760 bp in length, containing an ORF of 1,032 bp encoding 344 amino acids, with a conserved S-TKc region and a DVD region. As illustrated by amino acid alignment and phylogenetic tree, ChMKK6 shared the maximal (99.0%) identity showing MKK6 of Crassostrea gigas. As indicated by tissue expression analysis, ChMKK6 possessed the maximal expression within hepatopancreas, and was markedly increased in all tissues following Vibrio Parahaemolyticus (V. Parahaemolyticus) challenge. As stated by the qRT-PCR analysis, ChMKK6 silencing significantly suppressed IL17 transcription, suggesting its involvement in innate immunity by regulating IL17-mediated inflammatory response. Moreover, ChMKK6 silencing led to the suppression of key immune-related enzyme activities (ACP, AKP, CAT, LZM, and SOD), while raising the MDA content in gill. In addition, the antimicrobial viability of hemolymph supernatant was markedly reduced by ChMKK6 silencing. These results collectively demonstrate that ChMKK6 is a key player in immunity defense of C. hongkongensis from V. Parahaemolyticus.
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BibTeX
@article{zhu2026recognition,
title = {{Recognition and function analysis of MKK6 in immune reaction against Vibrio Parahaemolyticus in Crassostrea hongkongensis}},
author = {Jiexiong Zhu and Guohui Shao and Jiayu Chen and Jinji Lin and Qiwen Liang and Feifei Yu and Zhiwen Wang and Baixu Lin and Yongyao Huang and Huiyan Li and Junhui Xu},
journal = {PLOS One},
year = {2026},
month = oct,
doi = {10.1371/journal.pone.0360613},
url = {https://doi.org/10.1371/journal.pone.0360613},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Recognition and function analysis of MKK6 in immune reaction against Vibrio Parahaemolyticus in Crassostrea hongkongensis
AU - Jiexiong Zhu
AU - Guohui Shao
AU - Jiayu Chen
AU - Jinji Lin
AU - Qiwen Liang
AU - Feifei Yu
AU - Zhiwen Wang
AU - Baixu Lin
AU - Yongyao Huang
AU - Huiyan Li
AU - Junhui Xu
PY - 2026
DA - 2026/10/15
JO - PLOS One
DO - 10.1371/journal.pone.0360613
UR - https://doi.org/10.1371/journal.pone.0360613
AB - MKK6 is a key protein kinase that regulates inflammatory response under pathogen infection by activating MAPK signal pathway. However, the function of MKK6 remained unclear within oysters. Our research recognized a novel KK6 gene from C. hongkongensis, which is named ChMKK6. The cDNA of ChMKK6 gene was 2,760 bp in length, containing an ORF of 1,032 bp encoding 344 amino acids, with a conserved S-TKc region and a DVD region. As illustrated by amino acid alignment and phylogenetic tree, ChMKK6 shared the maximal (99.0%) identity showing MKK6 of Crassostrea gigas. As indicated by tissue expression analysis, ChMKK6 possessed the maximal expression within hepatopancreas, and was markedly increased in all tissues following Vibrio Parahaemolyticus (V. Parahaemolyticus) challenge. As stated by the qRT-PCR analysis, ChMKK6 silencing significantly suppressed IL17 transcription, suggesting its involvement in innate immunity by regulating IL17-mediated inflammatory response. Moreover, ChMKK6 silencing led to the suppression of key immune-related enzyme activities (ACP, AKP, CAT, LZM, and SOD), while raising the MDA content in gill. In addition, the antimicrobial viability of hemolymph supernatant was markedly reduced by ChMKK6 silencing. These results collectively demonstrate that ChMKK6 is a key player in immunity defense of C. hongkongensis from V. Parahaemolyticus.
SN - 1932-6203
ER -