Brassinosteroid receptors reveal conserved features of exon-embedded gene regulation
- 1University of Lausanne
Abstract
Precise quantitative and spatial control of plant gene expression is typically attributed to cis-regulatory elements in promoters and other non-coding sequences. The extent to which exon-encoded regulatory information shapes plant gene expression and how remains unclear. Here we used BRASSINOSTEROID INSENSITIVE 1 (BRI1) as a model locus and found that it contains an exonic enhancer (EE), which is essential to establish the spatial BRI1 expression pattern. Its disruption restricts BRI1 expression and impairs BRI1-dependent root growth, linking exon-encoded regulatory information and development. The BRI1 EE acts in concert with its promoter, and related receptor kinase genes likewise harbor regulatory information within their coding sequences. Genome-wide surveys show that candidate EEs are associated with highly connected chromatin interaction networks and transcriptionally active loci. Moreover, species comparisons suggest that common features of exon-encoded regulatory information extend across diverse plant lineages. Thus, exon-encoded regulatory information is a vital component of plant gene regulation.
Related
- Removal of seed coat H3K27me3 in Arabidopsis requires noncanonical functions of brassinosteroid receptors
- Post-Translational Regulation and Ubiquitination of Brassinosteroid Signaling Components in Plants
- Evolutionary Dynamics of the Complete Chemosensory Repertoire in Kissing Bugs of the Genus Rhodnius : Divergent Odorant Receptors Contrast with Conserved Gene Families
- Context-dependent regulation of cytotoxic T lymphocytes by nuclear receptors
- Genomic and Transcriptomic Analysis of Nine Resurrection Plant Species: Conserved Regulation Versus Structural Paralog Capacity in Desiccation Tolerance
BibTeX
@misc{deniamondejar2026brassinosteroid,
title = {{Brassinosteroid receptors reveal conserved features of exon-embedded gene regulation}},
author = {R. Denia-Mondejar and P. Brunot-Garau and X. Ma and A. Serrano-Mislata and M. Dolores Gomez and D. Latrasse and M. Benhamed and C. S. Hardtke and N. Blanco-Tourinan},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.21.753065},
url = {https://www.biorxiv.org/content/10.64898/2026.09.21.753065v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Brassinosteroid receptors reveal conserved features of exon-embedded gene regulation
AU - R. Denia-Mondejar
AU - P. Brunot-Garau
AU - X. Ma
AU - A. Serrano-Mislata
AU - M. Dolores Gomez
AU - D. Latrasse
AU - M. Benhamed
AU - C. S. Hardtke
AU - N. Blanco-Tourinan
PY - 2026
DA - 2026/09/21
PB - bioRxiv
DO - 10.64898/2026.09.21.753065
UR - https://www.biorxiv.org/content/10.64898/2026.09.21.753065v1
AB - Precise quantitative and spatial control of plant gene expression is typically attributed to cis-regulatory elements in promoters and other non-coding sequences. The extent to which exon-encoded regulatory information shapes plant gene expression and how remains unclear. Here we used BRASSINOSTEROID INSENSITIVE 1 (BRI1) as a model locus and found that it contains an exonic enhancer (EE), which is essential to establish the spatial BRI1 expression pattern. Its disruption restricts BRI1 expression and impairs BRI1-dependent root growth, linking exon-encoded regulatory information and development. The BRI1 EE acts in concert with its promoter, and related receptor kinase genes likewise harbor regulatory information within their coding sequences. Genome-wide surveys show that candidate EEs are associated with highly connected chromatin interaction networks and transcriptionally active loci. Moreover, species comparisons suggest that common features of exon-encoded regulatory information extend across diverse plant lineages. Thus, exon-encoded regulatory information is a vital component of plant gene regulation.
ER -