On the mechanism of antibiofilm activity of Distreptase® Distrept
- 1SI «Institute of Pharmacology and Toxicology of the NAMS of Ukraine», Kyiv, Ukraine
Abstract
Objective of the study: to study some mechanisms underlying the antibiofilm effect of the enzyme preparation Distreptaza® Distrept against Staphylococcus aureus and its effect on the specific antimicrobial activity of clindamycin. Materials and methods. methicillin-resistant clinical isolate of S. aureus 222 was used in the study. The study of the antibiofilm effect of the studied preparations was carried out according to the generally accepted method, which involves the sorption of gentian violet by biofilm structures, followed by desorption of the dye into an organic solvent. The influence of Distreptaza® Distrept and clindamycin on the expression of genes regulating biofilm formation (icaA, icaR), synthesis of matrix components (cidA), synthesis of fibrinogen-binding protein (fib) was assessed by real-time polymerase chain reaction. Results. The data obtained in the experiments indicate a pronounced antibiofilm activity of clindamycin and Distreptaza® Distrept, as well as the ability of the enzyme preparation to increase the specific antimicrobial effect of clindamycin. It has been established that an increase in the duration of drugs action is accompanied by an increase in the antibiofilm effect, after 3 days of exposure, the biofilm biomass is minimal (1.5%). Under the action of Distreptaza® Distrept increases the penetration of antimicrobial drugs through the biofilm matrix. Distreptaza® Distrept stimulates the expression of the cidA gene, and when clindamycin and Distreptaza® Distrept act on biofilms, the expression of the icaA gene is suppressed 3.6-fold. Distreptaza® Distrept affects the expression of the fib gene, changes in the expression of the fib gene were also detected during the action with clindamycin. There are no significant changes in the protein content in the biofilm matrix under the action of drugs. Conclusions. The enzyme drug Distreptaza® Distrept disrupted biofilm formation of methicillin-resistant S. aureus 222, enhanced the antibiofilm effect of clindamycin, and improved the penetration of antimicrobial drugs into the biofilm, which may increase the effectiveness of the treatment of chronic recurrent infections of the female reproductive tract.
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BibTeX
@article{vrynchanu2026mechanism,
title = {{On the mechanism of antibiofilm activity of Distreptase® Distrept}},
author = {N.O. Vrynchanu and V.V. Samsonova and N.I. Hrynchuk},
journal = {Репродуктивная эндокринология},
year = {2026},
month = jul,
doi = {10.18370/2309-4117.2026.84.33-40},
url = {https://doi.org/10.18370/2309-4117.2026.84.33-40},
note = {CC BY},
}
RIS
TY - JOUR
TI - On the mechanism of antibiofilm activity of Distreptase® Distrept
AU - N.O. Vrynchanu
AU - V.V. Samsonova
AU - N.I. Hrynchuk
PY - 2026
DA - 2026/07/01
JO - Репродуктивная эндокринология
DO - 10.18370/2309-4117.2026.84.33-40
UR - https://doi.org/10.18370/2309-4117.2026.84.33-40
AB - Objective of the study: to study some mechanisms underlying the antibiofilm effect of the enzyme preparation Distreptaza® Distrept against Staphylococcus aureus and its effect on the specific antimicrobial activity of clindamycin. Materials and methods. methicillin-resistant clinical isolate of S. aureus 222 was used in the study. The study of the antibiofilm effect of the studied preparations was carried out according to the generally accepted method, which involves the sorption of gentian violet by biofilm structures, followed by desorption of the dye into an organic solvent. The influence of Distreptaza® Distrept and clindamycin on the expression of genes regulating biofilm formation (icaA, icaR), synthesis of matrix components (cidA), synthesis of fibrinogen-binding protein (fib) was assessed by real-time polymerase chain reaction. Results. The data obtained in the experiments indicate a pronounced antibiofilm activity of clindamycin and Distreptaza® Distrept, as well as the ability of the enzyme preparation to increase the specific antimicrobial effect of clindamycin. It has been established that an increase in the duration of drugs action is accompanied by an increase in the antibiofilm effect, after 3 days of exposure, the biofilm biomass is minimal (1.5%). Under the action of Distreptaza® Distrept increases the penetration of antimicrobial drugs through the biofilm matrix. Distreptaza® Distrept stimulates the expression of the cidA gene, and when clindamycin and Distreptaza® Distrept act on biofilms, the expression of the icaA gene is suppressed 3.6-fold. Distreptaza® Distrept affects the expression of the fib gene, changes in the expression of the fib gene were also detected during the action with clindamycin. There are no significant changes in the protein content in the biofilm matrix under the action of drugs. Conclusions. The enzyme drug Distreptaza® Distrept disrupted biofilm formation of methicillin-resistant S. aureus 222, enhanced the antibiofilm effect of clindamycin, and improved the penetration of antimicrobial drugs into the biofilm, which may increase the effectiveness of the treatment of chronic recurrent infections of the female reproductive tract.
SN - 2309-4117
SN - 2411-1295
ER -