Quantification of Y RNAs in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.
- 1Post-Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, PR, Brazil.
- 2Immunogenetics Laboratory, Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Abstract
Objective
To investigate the expression of Y1, Y3, Y4, and Y5 in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.Methods
This case-control study included 10 patients with ankylosing spondylitis and 18 healthy controls from northern and northwestern Paraná, Brazil. The diagnosis of ankylosing spondylitis was confirmed by a rheumatologist according to the 2009 and 2011 ASAS criteria. Total RNA was extracted from peripheral blood using TRIzol reagent, treated with DNase I, and reverse-transcribed into cDNA. Y RNA expression was quantified by qPCR using SYBR™ Green, and the data were analyzed using Thermo Fisher Cloud.Results
Overall Y RNA expression did not differ significantly between patients and controls; however, lower ΔCt values, indicating higher Y1 and Y5 expression, were significantly associated with higher Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) scores. Pairwise comparisons showed that Y3 and Y4 were expressed at lower levels than Y1 and that Y4 expression was lower than Y3 and Y5 expression; no significant differences were observed between Y1 and Y5 or between Y3 and Y5 in either patients or controls.Conclusion
These findings suggest that Y1 and Y5 expression is associated with disease activity, although no statistically significant differences in overall Y RNA expression were observed between patients and controls.
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BibTeX
@article{castellani2026quantification,
title = {{Quantification of Y RNAs in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.}},
author = {Guilherme Castellani and Matheus Braga and Aléia Harumi Uchibaba Yamanaka and Giovana Paola Zaccarias Bemvides and Julyane Schavaren and Larissa Danielle Bahls Pinto and Quirino Alves de Lima Neto},
journal = {Einstein (Sao Paulo, Brazil)},
year = {2026},
month = oct,
doi = {10.31744/einstein_journal/2026ao2319},
url = {https://europepmc.org/article/MED/42848639},
note = {CC BY},
}
RIS
TY - JOUR
TI - Quantification of Y RNAs in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.
AU - Guilherme Castellani
AU - Matheus Braga
AU - Aléia Harumi Uchibaba Yamanaka
AU - Giovana Paola Zaccarias Bemvides
AU - Julyane Schavaren
AU - Larissa Danielle Bahls Pinto
AU - Quirino Alves de Lima Neto
PY - 2026
DA - 2026/10/05
JO - Einstein (Sao Paulo, Brazil)
DO - 10.31744/einstein_journal/2026ao2319
UR - https://europepmc.org/article/MED/42848639
AB - Objective To investigate the expression of Y1, Y3, Y4, and Y5 in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.Methods This case-control study included 10 patients with ankylosing spondylitis and 18 healthy controls from northern and northwestern Paraná, Brazil. The diagnosis of ankylosing spondylitis was confirmed by a rheumatologist according to the 2009 and 2011 ASAS criteria. Total RNA was extracted from peripheral blood using TRIzol reagent, treated with DNase I, and reverse-transcribed into cDNA. Y RNA expression was quantified by qPCR using SYBR™ Green, and the data were analyzed using Thermo Fisher Cloud.Results Overall Y RNA expression did not differ significantly between patients and controls; however, lower ΔCt values, indicating higher Y1 and Y5 expression, were significantly associated with higher Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) scores. Pairwise comparisons showed that Y3 and Y4 were expressed at lower levels than Y1 and that Y4 expression was lower than Y3 and Y5 expression; no significant differences were observed between Y1 and Y5 or between Y3 and Y5 in either patients or controls.Conclusion These findings suggest that Y1 and Y5 expression is associated with disease activity, although no statistically significant differences in overall Y RNA expression were observed between patients and controls.
SN - 1679-4508
SN - 2317-6385
ER -