NtMYB1 and NtbZIP53 antagonistically regulate NtGA3ox2 and NtGA2ox8 mediating diverse GA biosyntheses and tiny-plant in Narcissus tazetta
Abstract
Miniaturization and multiple branching are characteristic features of horticultural plants. Gibberellins participate in regulating the size and branching of plant architecture. Gibberellin 3β hydroxylase (GA3ox) and gibberellin 2β hydroxylase (GA2ox) are a pair of key enzymes for the homeostasis of active GA. However, the transcriptional regulation of GA3ox and GA2ox and the synthesis of active GAs remain unknown. Here the roles of narcissus NtMYB1 and NtbZIP53 in regulating the biosynthesis of diverse active GAs were addressed. Overexpression of NtMYB1 in Arabidopsis and Narcissus shows a tiny-type phenotype. The NtMYB1 co-expression network reveals that NtMYB1 or NtbZIP53 is closely related to NtKS1, NtGA20ox, NtGA2ox, and NtGA3ox in the GA biosynthetic pathway of narcissus. NtMYB1 can interact with NtbZIP53. Overexpression of NtMYB1 and NtMYB1/NtbZIP53 in transgenic narcissus upregulated the expression of upstream genes NtKS1 and NtGA20ox in the GA biosynthesis pathway; however, NtMYB1 and/or NtbZIP53 directly targeted the promoters of NtGA3ox2 and NtGA2ox8 and antagonistically controlled NtGA3ox2 and NtGA3ox8 transcription, respectively, and both decreased GA1 accumulation. NtMYB1 recruiting NtbZIP53 enhanced the repression of NtGA3ox2 and the activation of NtGA2ox8, reducing the GA1/GA3 ratio and plant size in narcissus. This provides a conceptual basis for controlling the architecture of Chinese narcissus.
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BibTeX
@article{wu2026ntmyb1,
title = {{NtMYB1 and NtbZIP53 antagonistically regulate NtGA3ox2 and NtGA2ox8 mediating diverse GA biosyntheses and tiny-plant in Narcissus tazetta}},
author = {Zhuoyu Wu and Xi Wu and Zheng Gu and Jingwen Yang and Yajue Chen and Yun Xu and Ruizhe Feng and Yu Zhang and Yujun Ren and Ying Miao},
journal = {Horticulture research},
year = {2026},
month = jun,
doi = {10.1093/hr/uhag234},
url = {https://europepmc.org/article/PMC/PMC13723081},
note = {CC BY},
}
RIS
TY - JOUR
TI - NtMYB1 and NtbZIP53 antagonistically regulate NtGA3ox2 and NtGA2ox8 mediating diverse GA biosyntheses and tiny-plant in Narcissus tazetta
AU - Zhuoyu Wu
AU - Xi Wu
AU - Zheng Gu
AU - Jingwen Yang
AU - Yajue Chen
AU - Yun Xu
AU - Ruizhe Feng
AU - Yu Zhang
AU - Yujun Ren
AU - Ying Miao
PY - 2026
DA - 2026/06/08
JO - Horticulture research
DO - 10.1093/hr/uhag234
UR - https://europepmc.org/article/PMC/PMC13723081
AB - Miniaturization and multiple branching are characteristic features of horticultural plants. Gibberellins participate in regulating the size and branching of plant architecture. Gibberellin 3β hydroxylase (GA3ox) and gibberellin 2β hydroxylase (GA2ox) are a pair of key enzymes for the homeostasis of active GA. However, the transcriptional regulation of GA3ox and GA2ox and the synthesis of active GAs remain unknown. Here the roles of narcissus NtMYB1 and NtbZIP53 in regulating the biosynthesis of diverse active GAs were addressed. Overexpression of NtMYB1 in Arabidopsis and Narcissus shows a tiny-type phenotype. The NtMYB1 co-expression network reveals that NtMYB1 or NtbZIP53 is closely related to NtKS1, NtGA20ox, NtGA2ox, and NtGA3ox in the GA biosynthetic pathway of narcissus. NtMYB1 can interact with NtbZIP53. Overexpression of NtMYB1 and NtMYB1/NtbZIP53 in transgenic narcissus upregulated the expression of upstream genes NtKS1 and NtGA20ox in the GA biosynthesis pathway; however, NtMYB1 and/or NtbZIP53 directly targeted the promoters of NtGA3ox2 and NtGA2ox8 and antagonistically controlled NtGA3ox2 and NtGA3ox8 transcription, respectively, and both decreased GA1 accumulation. NtMYB1 recruiting NtbZIP53 enhanced the repression of NtGA3ox2 and the activation of NtGA2ox8, reducing the GA1/GA3 ratio and plant size in narcissus. This provides a conceptual basis for controlling the architecture of Chinese narcissus.
SN - 2662-6810
SN - 2052-7276
ER -