Rapid diagnosis of human paragonimiasis using recombinant
4 affiliations
- 1Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand
- 2Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand
- 3Department of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan; Department of Tropical Medicine and Parasitology, School of Medicine, Juntendo University, Tokyo, Japan
- 4Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand; Corresponding author:
Abstract
Objectives: Cysteine proteases, the major components of excretory-secretory substances excreted from adult Paragonimus worms, are known to be useful diagnostic antigens for human paragonimiasis. In this study, we developed immunochromatography-based kits to detect specific immunoglobulin G (IgG) and immunoglobulin G4 (IgG4) antibodies against recombinant cysteine protease-6 (rCP-6) and evaluated their diagnostic performance. Methods: The rCP-6 was expressed in Escherichia coli , affinity-purified, and evaluated for immunoreactivity. The rCP-6-based immunochromatographic kits were evaluated for the detection of IgG and IgG4 using simulated whole-blood and serum samples from paragonimiasis patients, healthy individuals, and patients with other infectious diseases. The diagnostic performance was evaluated. Results: The IgG detection kit exhibited sensitivity of 76.7%, specificity of 90.5%, and accuracy of 87.3% for both simulated whole-blood and serum samples. In comparison, the IgG4 detection kit demonstrated higher performance, with 85.0% sensitivity, 100% specificity, and 96.5% accuracy across both sample types. Conclusion: Both IgG and IgG4 detection kits were useful for diagnosing paragonimiasis. The IgG4 detection kit showed superior diagnostic performance. These immunochromatographic tools may be applicable in clinical laboratories for diagnosing outpatients with paragonimiasis caused by different Paragonimus species ( P. heterotremus, P. westermani , and P. skrjabini miyazakii ).
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BibTeX
@article{boonroumkaew2026rapid,
title = {{Rapid diagnosis of human paragonimiasis using recombinant}},
author = {Patcharaporn Boonroumkaew and Lakkhana Sadaow and Nongnapas Kanchanangkul and Rutchanee Rodpai and Oranuch Sanpool and Pewpan Maleewong Intapan and Hiroshi Yamasaki and Wanchai Maleewong},
journal = {International Journal of Infectious Diseases},
year = {2026},
month = oct,
doi = {10.1016/j.ijid.2026.109098},
url = {https://doi.org/10.1016/j.ijid.2026.109098},
note = {CC BY},
}
RIS
TY - JOUR
TI - Rapid diagnosis of human paragonimiasis using recombinant
AU - Patcharaporn Boonroumkaew
AU - Lakkhana Sadaow
AU - Nongnapas Kanchanangkul
AU - Rutchanee Rodpai
AU - Oranuch Sanpool
AU - Pewpan Maleewong Intapan
AU - Hiroshi Yamasaki
AU - Wanchai Maleewong
PY - 2026
DA - 2026/10/30
JO - International Journal of Infectious Diseases
DO - 10.1016/j.ijid.2026.109098
UR - https://doi.org/10.1016/j.ijid.2026.109098
AB - Objectives: Cysteine proteases, the major components of excretory-secretory substances excreted from adult Paragonimus worms, are known to be useful diagnostic antigens for human paragonimiasis. In this study, we developed immunochromatography-based kits to detect specific immunoglobulin G (IgG) and immunoglobulin G4 (IgG4) antibodies against recombinant cysteine protease-6 (rCP-6) and evaluated their diagnostic performance. Methods: The rCP-6 was expressed in Escherichia coli , affinity-purified, and evaluated for immunoreactivity. The rCP-6-based immunochromatographic kits were evaluated for the detection of IgG and IgG4 using simulated whole-blood and serum samples from paragonimiasis patients, healthy individuals, and patients with other infectious diseases. The diagnostic performance was evaluated. Results: The IgG detection kit exhibited sensitivity of 76.7%, specificity of 90.5%, and accuracy of 87.3% for both simulated whole-blood and serum samples. In comparison, the IgG4 detection kit demonstrated higher performance, with 85.0% sensitivity, 100% specificity, and 96.5% accuracy across both sample types. Conclusion: Both IgG and IgG4 detection kits were useful for diagnosing paragonimiasis. The IgG4 detection kit showed superior diagnostic performance. These immunochromatographic tools may be applicable in clinical laboratories for diagnosing outpatients with paragonimiasis caused by different Paragonimus species ( P. heterotremus, P. westermani , and P. skrjabini miyazakii ).
SN - 1201-9712
SN - 1878-3511
ER -