Stigmatic papilla cells recover nonself pollen acceptance after self-pollen recognition
- 1University of Tokyo
Abstract
In Brassicaceae, self-incompatibility is initiated when the pollen ligand SP11/SCR activates the cognate stigma receptor kinase SRK. Although this interaction is usually considered at a single pollen-papilla interface, individual papilla cells encounter mixed pollen loads. Here, using a self-incompatibility-reconstituted Arabidopsis thaliana system, we show that self-pollen recognition transiently delays the hydration and germination of neighboring nonself pollen. The delay was stronger when two self-pollen grains were present but largely disappeared when nonself pollen was applied 2 h after self pollen. Repollination after removal of a self-pollen grain nevertheless revealed persistent inhibition at the original contact site. Live imaging with Lifeact-tdTomato showed that self-pollination transiently broadened F-actin orientations throughout the papilla cell, followed by recovery toward the longitudinal organization of unpollinated cells. Upon self- and non-self-dual pollination, actin-bundle orientation returned toward the papilla long axis prior to the delayed hydration of the non-self-pollen. These observations support the view that the nature of the self-recognition signaling produces a reversible, papilla-wide state that temporarily reduces compatible-pollen acceptance, while local self-rejection persists at the original contact site.
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BibTeX
@misc{inoue2026stigmatic,
title = {{Stigmatic papilla cells recover nonself pollen acceptance after self-pollen recognition}},
author = {Y. Inoue and S. Fujii},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.10.750793},
url = {https://www.biorxiv.org/content/10.64898/2026.09.10.750793v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Stigmatic papilla cells recover nonself pollen acceptance after self-pollen recognition
AU - Y. Inoue
AU - S. Fujii
PY - 2026
DA - 2026/09/10
PB - bioRxiv
DO - 10.64898/2026.09.10.750793
UR - https://www.biorxiv.org/content/10.64898/2026.09.10.750793v1
AB - In Brassicaceae, self-incompatibility is initiated when the pollen ligand SP11/SCR activates the cognate stigma receptor kinase SRK. Although this interaction is usually considered at a single pollen-papilla interface, individual papilla cells encounter mixed pollen loads. Here, using a self-incompatibility-reconstituted Arabidopsis thaliana system, we show that self-pollen recognition transiently delays the hydration and germination of neighboring nonself pollen. The delay was stronger when two self-pollen grains were present but largely disappeared when nonself pollen was applied 2 h after self pollen. Repollination after removal of a self-pollen grain nevertheless revealed persistent inhibition at the original contact site. Live imaging with Lifeact-tdTomato showed that self-pollination transiently broadened F-actin orientations throughout the papilla cell, followed by recovery toward the longitudinal organization of unpollinated cells. Upon self- and non-self-dual pollination, actin-bundle orientation returned toward the papilla long axis prior to the delayed hydration of the non-self-pollen. These observations support the view that the nature of the self-recognition signaling produces a reversible, papilla-wide state that temporarily reduces compatible-pollen acceptance, while local self-rejection persists at the original contact site.
ER -