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PreprintCC BY-NC-NDbioRxiv

roostR: An R package to examine diel activity patterns from Motus radio telemetry data

  1. 1Ohio University

Abstract

1. Monitoring the diel activity patterns of free-living animals is a methodological challenge. Signal strength fluctuations from Very High Frequency (VHF) radio transmitters deployed within the Motus Wildlife Tracking System can be used as a proxy for activity. However, analytical tools to extract behavioral metrics that quantify activity patterns from these data are needed. 2. We developed roostR, an open-source R package that converts Motus detection data into quantitative behavioral metrics, including roost initiation and departure, roost duration, observation time, and restlessness. The package uses a sequential pipeline built around signal volatility and rolling medians to detect transitions between active and inactive states. Default parameter values were tuned using data from 55 dark-eyed juncos (Junco hyemalis) overwintering in southeastern Ohio. 3. We provide an example from a dark-eyed junco over a 58-day period. roostR estimated roost onset on 48 nights and departure on 56 mornings, with higher rolling median signal differences during the day than at night, consistent with a diurnal animal, and variable restlessness periods each night. We also used the package to estimate roost behavior of an American tree sparrow (Spizelloides arborea) over 43-nights. 4. roostR enables researchers to extract individual activity data from Motus datasets. Because all thresholds are user-adjustable, the pipeline is adaptable across species, tag specifications, and ecological contexts, enabling researchers to test hypotheses about how environmental factors influence diel activity patterns.

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BibTeX
@misc{williams2026roostr,
  title = {{roostR: An R package to examine diel activity patterns from Motus radio telemetry data}},
  author = {K. A. Williams and J. E. Morgan and S. M. Lyons},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.10.750597},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.10.750597v1},
  note = {CC BY-NC-ND},
}
RIS
TY  - UNPB
TI  - roostR: An R package to examine diel activity patterns from Motus radio telemetry data
AU  - K. A. Williams
AU  - J. E. Morgan
AU  - S. M. Lyons
PY  - 2026
DA  - 2026/09/10
PB  - bioRxiv
DO  - 10.64898/2026.09.10.750597
UR  - https://www.biorxiv.org/content/10.64898/2026.09.10.750597v1
AB  - 1. Monitoring the diel activity patterns of free-living animals is a methodological challenge. Signal strength fluctuations from Very High Frequency (VHF) radio transmitters deployed within the Motus Wildlife Tracking System can be used as a proxy for activity. However, analytical tools to extract behavioral metrics that quantify activity patterns from these data are needed. 2. We developed roostR, an open-source R package that converts Motus detection data into quantitative behavioral metrics, including roost initiation and departure, roost duration, observation time, and restlessness. The package uses a sequential pipeline built around signal volatility and rolling medians to detect transitions between active and inactive states. Default parameter values were tuned using data from 55 dark-eyed juncos (Junco hyemalis) overwintering in southeastern Ohio. 3. We provide an example from a dark-eyed junco over a 58-day period. roostR estimated roost onset on 48 nights and departure on 56 mornings, with higher rolling median signal differences during the day than at night, consistent with a diurnal animal, and variable restlessness periods each night. We also used the package to estimate roost behavior of an American tree sparrow (Spizelloides arborea) over 43-nights. 4. roostR enables researchers to extract individual activity data from Motus datasets. Because all thresholds are user-adjustable, the pipeline is adaptable across species, tag specifications, and ecological contexts, enabling researchers to test hypotheses about how environmental factors influence diel activity patterns.
ER  -