Comparative analysis of 15 chromosome-scale near T2T assemblies of Brazilian Fusarium graminearum isolates
- 1Rothamsted Research
Abstract
Fusarium graminearum is the causative agent of Fusarium head blight of wheat, yet genomic resources for Brazilian populations, where it is the dominant species, remain limited to a single reference genome. We report fifteen near telomere-to-telomere (T2T) assemblies of Brazilian F. graminearum isolates generated with Oxford Nanopore sequencing. Each genome was assembled into four nuclear chromosomes, with 118 of 120 telomeres resolved, BUSCO completeness of 99.199.3%, and 14,80214,894 predicted genes per isolate. The genomes are structurally conserved, showing no chromosome-scale rearrangements and uniform transposable element content. Orthology assignment placed 90.4% of genes in the core genome. This suggests that the large phenotypic variance in aggressiveness observed between isolates likely resides in regulatory differences and/or within the small and largely uncharacterised accessory fraction. Ultimately, these assemblies provide a high-resolution resource for the global FHB community and a foundation for comparative analysis of a previously underrepresented population.
Related
- Fungistatic and non-fungistatic soils as reservoirs of bacteria antagonistic to Fusarium graminearum
- Effect of Photoperiod on Growth and Production of Trichothecene Mycotoxins in Fusarium graminearum.
- Deletion of Major Endoxylanase Genes in Fusarium graminearum Uncovers Distinct Virulence Phenotypes Influenced by the Genetic Background
- Sensitivity of Fusarium graminearum to propiconazole and potential resistance mechanisms.
- FgNup2 regulates nuclear import of the histone H2B monoubiquitination enzyme by stabilizing the FgImpα-FgBre1 complex to mediate pathogenicity in Fusarium graminearum
BibTeX
@misc{kroll2026comparative,
title = {{Comparative analysis of 15 chromosome-scale near T2T assemblies of Brazilian Fusarium graminearum isolates}},
author = {E. Kroll and A. M. Wood and C. P. Nicolli and A. de Klerk and M. Urban and E. Del Ponte and K. Hassani-Pak and K. E. Hammond-Kosack},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.16.752100},
url = {https://www.biorxiv.org/content/10.64898/2026.09.16.752100v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Comparative analysis of 15 chromosome-scale near T2T assemblies of Brazilian Fusarium graminearum isolates
AU - E. Kroll
AU - A. M. Wood
AU - C. P. Nicolli
AU - A. de Klerk
AU - M. Urban
AU - E. Del Ponte
AU - K. Hassani-Pak
AU - K. E. Hammond-Kosack
PY - 2026
DA - 2026/09/16
PB - bioRxiv
DO - 10.64898/2026.09.16.752100
UR - https://www.biorxiv.org/content/10.64898/2026.09.16.752100v1
AB - Fusarium graminearum is the causative agent of Fusarium head blight of wheat, yet genomic resources for Brazilian populations, where it is the dominant species, remain limited to a single reference genome. We report fifteen near telomere-to-telomere (T2T) assemblies of Brazilian F. graminearum isolates generated with Oxford Nanopore sequencing. Each genome was assembled into four nuclear chromosomes, with 118 of 120 telomeres resolved, BUSCO completeness of 99.199.3%, and 14,80214,894 predicted genes per isolate. The genomes are structurally conserved, showing no chromosome-scale rearrangements and uniform transposable element content. Orthology assignment placed 90.4% of genes in the core genome. This suggests that the large phenotypic variance in aggressiveness observed between isolates likely resides in regulatory differences and/or within the small and largely uncharacterised accessory fraction. Ultimately, these assemblies provide a high-resolution resource for the global FHB community and a foundation for comparative analysis of a previously underrepresented population.
ER -