Influence of Gliricidia sepium living support on rhizosphere bacterial recruitment and fruit metabolomics in black pepper (Piper nigrum).
10 affiliations
- 1Socioenvironmental and Water Resources Institute, Federal Rural University of the Amazon - UFRA, Belém, Pará, Brazil.
- 2Laboratory of Simulation and Computational Biology, High Performing Computer Center, Federal University of Pará - UFPA, Belém, Pará, Brazil.
- 3Laboratory of Bioinformatics and Genomics of Microorganisms, Institute of Biological Science, Federal University of Pará - UFPA, Belém, Pará, Brazil.
- 4Institute of Biological Science, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil.
- 5Laboratório de Bioprospecção e Inovação Tecnológica de Produtos Naturais da Amazônia, Federal University of Pará - UFPA, Belém, Pará, Brazil.
- 6Amazon Integrated Metabolomics Center, Institute of Exact and Natural Sciences, Federal University of Pará - UFPA, Belém, Brazil.
- 7Laboratório de Bioprospecção e Inovação Tecnológica de Produtos Naturais da Amazônia, Federal University of Pará - UFPA, Belém, Pará, Brazil. [email protected].
- 8Socioenvironmental and Water Resources Institute, Federal Rural University of the Amazon - UFRA, Belém, Pará, Brazil. [email protected].
- 9Laboratory of Simulation and Computational Biology, High Performing Computer Center, Federal University of Pará - UFPA, Belém, Pará, Brazil. [email protected].
- 10Laboratory of Bioinformatics and Genomics of Microorganisms, Institute of Biological Science, Federal University of Pará - UFPA, Belém, Pará, Brazil. [email protected].
Abstract
The ability of the rhizosphere to recruit beneficial microorganisms is central to the sustainable management of crops. Here, we investigated, through shotgun metagenomic sequencing and mass spectrometry techniques, the microbial composition, antimicrobial resistance (AMRs), and metabolomic profiles of Piper nigrum cultivated under two support systems: living Gliricidia sepium support (SL/RL) and dead wooden support (SD/RD). Bulk and rhizosphere soils were sequenced to assess taxonomic structure and ARGs, with emphasis on Actinobacteria due to their relevance in antibiotic production. Actinobacteria were highly represented across the samples (~ 35%). Dead-support rhizospheres exhibited a broader ARG repertoire and greater abundance of taxa known to include antibiotic-producing members, whereas living-support rhizospheres showed reduced ARG diversity. Metabolomic analysis further revealed support-dependent physiological responses in the host plant. PLS-DA distinguished the two cultivation systems, and three defense-related flavonoids, such as sakuranetin, isovitexin, and orientin, which were enriched in fruits of plants grown with dead support, indicating activation of stress - and defense - associated metabolic pathways. Their low abundance in living-support plants suggests reduced physiological stress, likely linked to improved nutrient cycling and rhizosphere stability provided by G. sepium. However, the volatile and total phenolic profile did not display significant chemical alterations in the samples. Together, these results suggest that support type influences both microbial functions and plant secondary metabolism. Dead support creates a more stressful ecological context that enriches antimicrobial resistance genes ARGs and defense metabolites, whereas living support promotes a more balanced soil-plant-microbe environment, supporting the use of G. sepium as a sustainable and biologically advantageous cultivation strategy for black pepper.
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BibTeX
@article{chaves2026influence,
title = {{Influence of Gliricidia sepium living support on rhizosphere bacterial recruitment and fruit metabolomics in black pepper (Piper nigrum).}},
author = {Valéria Alves Chaves and Oscar Victor Cardenas Alegria and Carlos Willian Dias Dantas and Victor Benedito Costa Ferreira and David Tavares Martins and Rhodin Clarentz Joseph and Laís Sousa Almeida and Sandro Duarte de Souza and Paulo Wender P Gomes and Joyce Kelly R da Silva and Rommel Thiago Jucá Ramos},
journal = {World journal of microbiology \& biotechnology},
year = {2026},
month = oct,
doi = {10.1007/s11274-026-05278-1},
url = {https://europepmc.org/article/MED/42963482},
note = {CC BY},
}
RIS
TY - JOUR
TI - Influence of Gliricidia sepium living support on rhizosphere bacterial recruitment and fruit metabolomics in black pepper (Piper nigrum).
AU - Valéria Alves Chaves
AU - Oscar Victor Cardenas Alegria
AU - Carlos Willian Dias Dantas
AU - Victor Benedito Costa Ferreira
AU - David Tavares Martins
AU - Rhodin Clarentz Joseph
AU - Laís Sousa Almeida
AU - Sandro Duarte de Souza
AU - Paulo Wender P Gomes
AU - Joyce Kelly R da Silva
AU - Rommel Thiago Jucá Ramos
PY - 2026
DA - 2026/10/31
JO - World journal of microbiology & biotechnology
DO - 10.1007/s11274-026-05278-1
UR - https://europepmc.org/article/MED/42963482
AB - The ability of the rhizosphere to recruit beneficial microorganisms is central to the sustainable management of crops. Here, we investigated, through shotgun metagenomic sequencing and mass spectrometry techniques, the microbial composition, antimicrobial resistance (AMRs), and metabolomic profiles of Piper nigrum cultivated under two support systems: living Gliricidia sepium support (SL/RL) and dead wooden support (SD/RD). Bulk and rhizosphere soils were sequenced to assess taxonomic structure and ARGs, with emphasis on Actinobacteria due to their relevance in antibiotic production. Actinobacteria were highly represented across the samples (~ 35%). Dead-support rhizospheres exhibited a broader ARG repertoire and greater abundance of taxa known to include antibiotic-producing members, whereas living-support rhizospheres showed reduced ARG diversity. Metabolomic analysis further revealed support-dependent physiological responses in the host plant. PLS-DA distinguished the two cultivation systems, and three defense-related flavonoids, such as sakuranetin, isovitexin, and orientin, which were enriched in fruits of plants grown with dead support, indicating activation of stress - and defense - associated metabolic pathways. Their low abundance in living-support plants suggests reduced physiological stress, likely linked to improved nutrient cycling and rhizosphere stability provided by G. sepium. However, the volatile and total phenolic profile did not display significant chemical alterations in the samples. Together, these results suggest that support type influences both microbial functions and plant secondary metabolism. Dead support creates a more stressful ecological context that enriches antimicrobial resistance genes ARGs and defense metabolites, whereas living support promotes a more balanced soil-plant-microbe environment, supporting the use of G. sepium as a sustainable and biologically advantageous cultivation strategy for black pepper.
SN - 1573-0972
SN - 0959-3993
ER -