Hierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana.
- 1School of Biosciences, University of Nottingham, Loughborough, UK.
- 2School of Chemistry, University of Nottingham, University Park, Nottingham, UK.
- 3School of Biosciences, University of Nottingham, Loughborough, UK. [email protected].
Abstract
The sensing of molecular oxygen is an essential component of development and environmental response in both animals and plants. Few substrates of the plant oxygen-sensing PLANT CYSTEINE OXIDASE (PCO) N-degron pathway have been identified. Here we show that HYPOXIA TRANSDUCER1 (HYT1, previously named HYPOXIA-RESPONSIVE UNKNOWN PROTEIN, HUP40), an angiosperm-specific tail anchored-domain protein, is a substrate of this pathway through a Cys-2 dependent N-degron. HYT1 is stabilised in response to acute hypoxia in root tissues and is associated with the endoplasmic reticulum and nucleus. Genetic evidence shows that HYT1 is an important component in the transduction of oxygen-sensing, required for tolerance to acute hypoxia and other stress responses. Expression of HYT1 is directly activated by group VII ETHYLENE RESPONSE FACTOR transcription factors (ERFVIIs), that are also pathway substrates. The relationship between ERFVIIs and HYT1 has properties of a coherent feed-forward loop initiated and maintained by hypoxia, providing a mechanism to ensure robust response to reduced oxygen.
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BibTeX
@article{manriquegil2026hierarchical,
title = {{Hierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana.}},
author = {Isabel Manrique-Gil and Charlene Dambire and Susana Ubeda-Tomás and Gunjan Sharma and Mohamad Abbas and Cristina Sousa Correia and Andrea Bombana and Ranjan Swarup and Nicholas J Mitchell and Neil J Oldham and Michael J Holdsworth},
journal = {Nature communications},
year = {2026},
month = sep,
doi = {10.1038/s41467-026-77710-x},
url = {https://europepmc.org/article/MED/42881157},
note = {CC BY},
}
RIS
TY - JOUR
TI - Hierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana.
AU - Isabel Manrique-Gil
AU - Charlene Dambire
AU - Susana Ubeda-Tomás
AU - Gunjan Sharma
AU - Mohamad Abbas
AU - Cristina Sousa Correia
AU - Andrea Bombana
AU - Ranjan Swarup
AU - Nicholas J Mitchell
AU - Neil J Oldham
AU - Michael J Holdsworth
PY - 2026
DA - 2026/09/16
JO - Nature communications
DO - 10.1038/s41467-026-77710-x
UR - https://europepmc.org/article/MED/42881157
AB - The sensing of molecular oxygen is an essential component of development and environmental response in both animals and plants. Few substrates of the plant oxygen-sensing PLANT CYSTEINE OXIDASE (PCO) N-degron pathway have been identified. Here we show that HYPOXIA TRANSDUCER1 (HYT1, previously named HYPOXIA-RESPONSIVE UNKNOWN PROTEIN, HUP40), an angiosperm-specific tail anchored-domain protein, is a substrate of this pathway through a Cys-2 dependent N-degron. HYT1 is stabilised in response to acute hypoxia in root tissues and is associated with the endoplasmic reticulum and nucleus. Genetic evidence shows that HYT1 is an important component in the transduction of oxygen-sensing, required for tolerance to acute hypoxia and other stress responses. Expression of HYT1 is directly activated by group VII ETHYLENE RESPONSE FACTOR transcription factors (ERFVIIs), that are also pathway substrates. The relationship between ERFVIIs and HYT1 has properties of a coherent feed-forward loop initiated and maintained by hypoxia, providing a mechanism to ensure robust response to reduced oxygen.
SN - 2041-1723
ER -