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Peer-reviewedCC BY 4.0Biomolecules

Detection of N4-Acetylcytosine in Escherichia coli Using APOBEC3A-Mediated Deamination Sequencing

  • Yaqian Liu1
  • Xin Xi1
  • Xiaocheng Weng1
  • Xiang Zhou1
  • Yuhao Du1
  1. 1Key Laboratory of Biomedical Polymers-Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
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Abstract

N4-acetylcytosine (4acC) is a naturally occurring DNA modification; however, its presence and distribution characteristics in prokaryotes remain unclear. In this study, based on the differential deamination activity of APOBEC3A (A3A) toward cytosine (C) and 4acC, we developed a single-base-resolution sequencing method termed 4acC-A3A-seq (4acC-APOBEC3A-sequencing). Following validation using template strands and optimization of reaction conditions, we applied this method to the genome of Escherichia coli (E. coli) and successfully identified 2271 high-confidence candidate 4acC sites, generating the first single-base-resolution genome-wide map of 4acC in a prokaryote. This study provided a key technological approach and foundational data for further exploration of the biological functions and evolutionary conservation of 4acC at single-base resolution.

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Cite

BibTeXRIS
BibTeX
@article{liu2026detection,
  title = {{Detection of N4-Acetylcytosine in Escherichia coli Using APOBEC3A-Mediated Deamination Sequencing}},
  author = {Yaqian Liu and Xin Xi and Xiaocheng Weng and Xiang Zhou and Yuhao Du},
  journal = {Biomolecules},
  year = {2026},
  month = oct,
  doi = {10.3390/biom16111570},
  url = {https://www.mdpi.com/2218-273X/16/11/1570},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Detection of N4-Acetylcytosine in Escherichia coli Using APOBEC3A-Mediated Deamination Sequencing
AU  - Yaqian Liu
AU  - Xin Xi
AU  - Xiaocheng Weng
AU  - Xiang Zhou
AU  - Yuhao Du
PY  - 2026
DA  - 2026/10/28
JO  - Biomolecules
DO  - 10.3390/biom16111570
UR  - https://www.mdpi.com/2218-273X/16/11/1570
AB  - N4-acetylcytosine (4acC) is a naturally occurring DNA modification; however, its presence and distribution characteristics in prokaryotes remain unclear. In this study, based on the differential deamination activity of APOBEC3A (A3A) toward cytosine (C) and 4acC, we developed a single-base-resolution sequencing method termed 4acC-A3A-seq (4acC-APOBEC3A-sequencing). Following validation using template strands and optimization of reaction conditions, we applied this method to the genome of Escherichia coli (E. coli) and successfully identified 2271 high-confidence candidate 4acC sites, generating the first single-base-resolution genome-wide map of 4acC in a prokaryote. This study provided a key technological approach and foundational data for further exploration of the biological functions and evolutionary conservation of 4acC at single-base resolution.
SN  - 2218-273X
ER  -