Isolation and pathogenicity characterization of a tembusu virus from 817 broilers with decreased egg production
- 1College of Veterinary Medicine, Shandong Agricultural University, Taian, 271017, China
- 2Shandong Provincial Key Laboratory of Zoonoses, Shandong, Taian, 271017, China
Abstract
Tembusu virus (TMUV) is widely distributed in duck and goose populations in Asia, causing significant economic losses to the poultry industry. In this study, a TMUV strain, designated 24SD, was isolated and characterized from 817 broilers with decreased egg production in Shandong province of China, in 2024. The 24SD replicated efficiently in BHK-21 cells and SPF chicken embryos, reaching titers 106.833 TCID₅₀ per 0.1 mL and 103.681 ELD₅₀ per 0.3 mL, respectively. The virus also replicated in duck embryos and caused obvious pathological lesions. Whole-genome sequencing (WGS) and phylogenetic analysis indicated that 24SD belongs to TMUV Cluster 3. The 24SD strain showed high nucleotide sequence identities (99.00-99.03 %) with other Cluster 3 strains, including YN2020, CTLN, and SD2021. Animal pathogenicity studies demonstrated that 24SD could infect both chickens and ducklings, with stronger replication in chickens. The virus induced transient viremia, replicated efficiently in multiple organs, including the heart and liver, leading to varying degrees of tissue damage and inflammation, and was excreted via feces. Overall, this study expands the genomic dataset of chicken-origin TMUV strains within Cluster 3 and provides valuable information for surveillance and control of TMUV in poultry.
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BibTeX
@article{zhao2026isolation,
title = {{Isolation and pathogenicity characterization of a tembusu virus from 817 broilers with decreased egg production}},
author = {MingRui Zhao and YuTang Xie and Lei Bei and Chenchen Feng and Ruihua Zhang and Yu Meng and Yanli Zhu and Liangmeng Wei and Shijin Jiang},
journal = {Poultry Science},
year = {2026},
month = oct,
doi = {10.1016/j.psj.2026.108006},
url = {https://doi.org/10.1016/j.psj.2026.108006},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Isolation and pathogenicity characterization of a tembusu virus from 817 broilers with decreased egg production
AU - MingRui Zhao
AU - YuTang Xie
AU - Lei Bei
AU - Chenchen Feng
AU - Ruihua Zhang
AU - Yu Meng
AU - Yanli Zhu
AU - Liangmeng Wei
AU - Shijin Jiang
PY - 2026
DA - 2026/10/21
JO - Poultry Science
DO - 10.1016/j.psj.2026.108006
UR - https://doi.org/10.1016/j.psj.2026.108006
AB - Tembusu virus (TMUV) is widely distributed in duck and goose populations in Asia, causing significant economic losses to the poultry industry. In this study, a TMUV strain, designated 24SD, was isolated and characterized from 817 broilers with decreased egg production in Shandong province of China, in 2024. The 24SD replicated efficiently in BHK-21 cells and SPF chicken embryos, reaching titers 106.833 TCID₅₀ per 0.1 mL and 103.681 ELD₅₀ per 0.3 mL, respectively. The virus also replicated in duck embryos and caused obvious pathological lesions. Whole-genome sequencing (WGS) and phylogenetic analysis indicated that 24SD belongs to TMUV Cluster 3. The 24SD strain showed high nucleotide sequence identities (99.00-99.03 %) with other Cluster 3 strains, including YN2020, CTLN, and SD2021. Animal pathogenicity studies demonstrated that 24SD could infect both chickens and ducklings, with stronger replication in chickens. The virus induced transient viremia, replicated efficiently in multiple organs, including the heart and liver, leading to varying degrees of tissue damage and inflammation, and was excreted via feces. Overall, this study expands the genomic dataset of chicken-origin TMUV strains within Cluster 3 and provides valuable information for surveillance and control of TMUV in poultry.
SN - 0032-5791
SN - 1525-3171
ER -