Thermoregulatory roles of preoptic area histamine H1 receptor-expressing neurons
Abstract
The preoptic area (POA) is a key regulator of body temperature, orchestrating behavioral and physiological thermoregulatory functions, including fever, torpor, and homeostatic responses to ambient temperature. Histamine regulates body temperature across animal taxa, including mammals, lower vertebrates, and invertebrates. Hrh1, which encodes the histamine H1 receptor, is expressed in several neuronal populations in the POA with limited overlap with other POA thermoregulatory neurons. Acute activation of POAHrh1 neurons increased body temperature and physical activity. Acute inhibition attenuated the dark phase increase in body temperature without affecting physical activity. There was no effect of inhibition on body temperature during the light phase, fever, cold exposure, or warm exposure. Assessing behavioral thermoregulation, we found that POAHrh1 neurons stimulated nest building in both sexes, possibly contributing to cold-induced nesting behavior. Through projections to the arcuate, dorsomedial hypothalamic and tuberomammillary nuclei, POAHrh1 neurons can increase body temperature and physical activity. Overall, POAHrh1 neurons participate in several thermoregulatory functions, including daily body temperature rhythm regulation, physical activity, and nest building behavior.
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BibTeX
@misc{pinol2026thermoregulatory,
title = {{Thermoregulatory roles of preoptic area histamine H1 receptor-expressing neurons}},
author = {R. A. Pinol and A. Valeri and C. Xiao and C. Hadley and A. I. Goldschmidt and D. Kerspern and S. Kulkarni and O. Gavrilova and A. Lutas and M. J. Krashes and M. Reitman},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.22.753241},
url = {https://www.biorxiv.org/content/10.64898/2026.09.22.753241v1},
note = {CC0},
}
RIS
TY - UNPB
TI - Thermoregulatory roles of preoptic area histamine H1 receptor-expressing neurons
AU - R. A. Pinol
AU - A. Valeri
AU - C. Xiao
AU - C. Hadley
AU - A. I. Goldschmidt
AU - D. Kerspern
AU - S. Kulkarni
AU - O. Gavrilova
AU - A. Lutas
AU - M. J. Krashes
AU - M. Reitman
PY - 2026
DA - 2026/09/22
PB - bioRxiv
DO - 10.64898/2026.09.22.753241
UR - https://www.biorxiv.org/content/10.64898/2026.09.22.753241v1
AB - The preoptic area (POA) is a key regulator of body temperature, orchestrating behavioral and physiological thermoregulatory functions, including fever, torpor, and homeostatic responses to ambient temperature. Histamine regulates body temperature across animal taxa, including mammals, lower vertebrates, and invertebrates. Hrh1, which encodes the histamine H1 receptor, is expressed in several neuronal populations in the POA with limited overlap with other POA thermoregulatory neurons. Acute activation of POAHrh1 neurons increased body temperature and physical activity. Acute inhibition attenuated the dark phase increase in body temperature without affecting physical activity. There was no effect of inhibition on body temperature during the light phase, fever, cold exposure, or warm exposure. Assessing behavioral thermoregulation, we found that POAHrh1 neurons stimulated nest building in both sexes, possibly contributing to cold-induced nesting behavior. Through projections to the arcuate, dorsomedial hypothalamic and tuberomammillary nuclei, POAHrh1 neurons can increase body temperature and physical activity. Overall, POAHrh1 neurons participate in several thermoregulatory functions, including daily body temperature rhythm regulation, physical activity, and nest building behavior.
ER -