Expression and Co-Expression Patterns of Selected MicroRNAs and ADAM Genes in Colorectal Cancer
4 affiliations
- 1Department of Metabolic Disease Prevention, Faculty of Public Health in Bytom, Medical University of Silesia in Katowice, 40-055 Katowice, Poland
- 2Department of Clinical Oncology, No. 4 Provincial Specialist Hospital, 41-902 Bytom, Poland
- 3Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 41-808 Zabrze, Poland
- 4Department of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 41-514 Katowice, Poland
Abstract
Colorectal cancer (CRC) is a leading cause of cancer-related death worldwide, and its molecular pathogenesis is not fully understood. This exploratory study investigated the expression of three selected microRNAs in colorectal cancer and their associations with previously characterized ADAM transcripts. RT-qPCR was used to assess miR-125b-5p, miR-338-3p, and miR-30c-5p in paired tumor and histologically tumor-free surgical margin tissues from 67 patients. Previously obtained ADAM10, ADAM12, ADAM17, and ADAM28 transcript data from the same cohort were included in correlation analyses. The Wilcoxon signed-rank test and Spearman’s rank correlation were applied, with Benjamini–Hochberg adjustment for multiple testing. MiR-125b-5p expression was lower in tumors than in matched margins (median 0.46 versus 1.33; adjusted p = 0.035). No significant paired differences were identified for miR-338-3p or miR-30c-5p. Margin miR-125b-5p expression was associated with neoadjuvant-treatment history (adjusted p = 0.033), although the treated subgroup was small. No miRNA-ADAM transcript correlations remained significant after adjustment. Positive correlations among some miRNA pairs were observed within tumor and margin tissues. The reduced level of relative miR-125b-5p expression in colorectal cancers is an observational finding consistent with the tumor-suppressor functions described in the literature, but it does not constitute proof of those functions. Inter-miRNA correlations do not demonstrate shared regulatory mechanisms, and the absence of miRNA-ADAM transcript associations does not exclude post-transcriptional regulation.
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BibTeX
@article{kalita2026expression,
title = {{Expression and Co-Expression Patterns of Selected MicroRNAs and ADAM Genes in Colorectal Cancer}},
author = {Agnieszka Kalita and Magdalena Sikora-Skrabaka and Karolina Gołąbek and Krzysztof Biernacki and Joanna Katarzyna Strzelczyk and Dariusz Waniczek and Ewa Nowakowska-Zajdel},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = nov,
doi = {10.3390/ijms27219983},
url = {https://www.mdpi.com/1422-0067/27/21/9983},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Expression and Co-Expression Patterns of Selected MicroRNAs and ADAM Genes in Colorectal Cancer
AU - Agnieszka Kalita
AU - Magdalena Sikora-Skrabaka
AU - Karolina Gołąbek
AU - Krzysztof Biernacki
AU - Joanna Katarzyna Strzelczyk
AU - Dariusz Waniczek
AU - Ewa Nowakowska-Zajdel
PY - 2026
DA - 2026/11/07
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219983
UR - https://www.mdpi.com/1422-0067/27/21/9983
AB - Colorectal cancer (CRC) is a leading cause of cancer-related death worldwide, and its molecular pathogenesis is not fully understood. This exploratory study investigated the expression of three selected microRNAs in colorectal cancer and their associations with previously characterized ADAM transcripts. RT-qPCR was used to assess miR-125b-5p, miR-338-3p, and miR-30c-5p in paired tumor and histologically tumor-free surgical margin tissues from 67 patients. Previously obtained ADAM10, ADAM12, ADAM17, and ADAM28 transcript data from the same cohort were included in correlation analyses. The Wilcoxon signed-rank test and Spearman’s rank correlation were applied, with Benjamini–Hochberg adjustment for multiple testing. MiR-125b-5p expression was lower in tumors than in matched margins (median 0.46 versus 1.33; adjusted p = 0.035). No significant paired differences were identified for miR-338-3p or miR-30c-5p. Margin miR-125b-5p expression was associated with neoadjuvant-treatment history (adjusted p = 0.033), although the treated subgroup was small. No miRNA-ADAM transcript correlations remained significant after adjustment. Positive correlations among some miRNA pairs were observed within tumor and margin tissues. The reduced level of relative miR-125b-5p expression in colorectal cancers is an observational finding consistent with the tumor-suppressor functions described in the literature, but it does not constitute proof of those functions. Inter-miRNA correlations do not demonstrate shared regulatory mechanisms, and the absence of miRNA-ADAM transcript associations does not exclude post-transcriptional regulation.
SN - 1422-0067
ER -