Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch
- 1Department of Cell Biology, Faculty of Science, Charles University, Praha, Czech Republic
Abstract
Ribosomal proteins, because of their RNA-binding capacity, may engage various cellular RNAs and fulfill non-ribosomal roles. Previously, we and others described the intergenic regulation mediated by splicing of RPL22 paralogs in Saccharomyces cerevisiae. Here, we prepared a panel of RPL22A/B intronic mutants with respect to their RNAfold-predicted features and analyzed their properties. We tested splicing efficiency and Rpl22-intron binding using an intron-containing reporter and a yeast three-hybrid system, respectively. We found that the splicing of RPL22 introns can be inhibited by stabilizing a predicted stem as part of a particular type of conformation (I structure). Stabilizing the formation of an alternate stem (P structure) permitted splicing. Binding of the Rpl22 protein to the intron led to enhanced splicing inhibition in WT and several of the mutants, which we interpret as stabilization of the I structure. Mutagenesis identified both the main and alternative 5’ss and additional stem loops as part of the regulatory mechanism. The inhibitory conformation of the intron did not prevent recognition of the 5’ss and branch point, but rather stalled splicing at a later stage, before the first catalytic step. We conclude that the structural ensemble of the RPL22 pre-mRNA behaves as an allosteric switch that responds to Rpl22 concentration.
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BibTeX
@article{abrhamova2026intronic,
title = {{Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch}},
author = {Kateřina Abrhámová and Alexandra Gredová and Karolína Navrátilová and Mohamed Boumaiza and Petr Folk},
journal = {PLOS One},
year = {2026},
month = oct,
doi = {10.1371/journal.pone.0359197},
url = {https://doi.org/10.1371/journal.pone.0359197},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch
AU - Kateřina Abrhámová
AU - Alexandra Gredová
AU - Karolína Navrátilová
AU - Mohamed Boumaiza
AU - Petr Folk
PY - 2026
DA - 2026/10/01
JO - PLOS One
DO - 10.1371/journal.pone.0359197
UR - https://doi.org/10.1371/journal.pone.0359197
AB - Ribosomal proteins, because of their RNA-binding capacity, may engage various cellular RNAs and fulfill non-ribosomal roles. Previously, we and others described the intergenic regulation mediated by splicing of RPL22 paralogs in Saccharomyces cerevisiae. Here, we prepared a panel of RPL22A/B intronic mutants with respect to their RNAfold-predicted features and analyzed their properties. We tested splicing efficiency and Rpl22-intron binding using an intron-containing reporter and a yeast three-hybrid system, respectively. We found that the splicing of RPL22 introns can be inhibited by stabilizing a predicted stem as part of a particular type of conformation (I structure). Stabilizing the formation of an alternate stem (P structure) permitted splicing. Binding of the Rpl22 protein to the intron led to enhanced splicing inhibition in WT and several of the mutants, which we interpret as stabilization of the I structure. Mutagenesis identified both the main and alternative 5’ss and additional stem loops as part of the regulatory mechanism. The inhibitory conformation of the intron did not prevent recognition of the 5’ss and branch point, but rather stalled splicing at a later stage, before the first catalytic step. We conclude that the structural ensemble of the RPL22 pre-mRNA behaves as an allosteric switch that responds to Rpl22 concentration.
SN - 1932-6203
ER -