Collagen4 fluorescent protein knock-in lines reveal remodeling of cardiovascular basement membranes
Abstract
Basement membranes (BMs) are specialized extracellular matrix structures that provide structural support and regulate cellular behavior. Although BM composition and organization are dynamically remodeled during development and tissue regeneration, the remodeling of individual BM components in the cardiovascular system (CVS) remain incompletely characterized. Here, we focused on Collagen4 (Col4), a major structural component of BMs, to investigate its remodeling during cardiovascular development and regeneration. To monitor the remodeling of Col4 in vivo, the Venus fluorescent tag was integrated in frame into the zebrafish endogenous col4a1 and col4a2 genes, enabling live confocal imaging of expression. Our data reveal dynamic patterns of Col4a1 and Col4a2 within the developing CVS, with deposition progressively increasing as vascular and cardiac structures develop and mature during both development and regeneration. In the regenerating heart, Col4a2 accumulated at the injury site during early stages of regeneration and became increasingly associated with the newly regenerated myocardium. Together, these findings demonstrate that Col4 deposition is dynamically reorganized across developmental and regenerative stages of CVS and describe new tools for following Col4 dynamics in vivo.
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BibTeX
@misc{tuladhar2026collagen4,
title = {{Collagen4 fluorescent protein knock-in lines reveal remodeling of cardiovascular basement membranes}},
author = {Astha Tuladhar and Elizabeth J Wunderlich and Shawn A Slaymaker and Aishwarya Pawar and Maura McGrail and Pascal Lafontant and Jeffrey J Essner},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.29.762927},
url = {https://www.biorxiv.org/content/10.64898/2026.10.29.762927v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Collagen4 fluorescent protein knock-in lines reveal remodeling of cardiovascular basement membranes
AU - Astha Tuladhar
AU - Elizabeth J Wunderlich
AU - Shawn A Slaymaker
AU - Aishwarya Pawar
AU - Maura McGrail
AU - Pascal Lafontant
AU - Jeffrey J Essner
PY - 2026
DA - 2026/10/29
PB - bioRxiv
DO - 10.64898/2026.10.29.762927
UR - https://www.biorxiv.org/content/10.64898/2026.10.29.762927v1
AB - Basement membranes (BMs) are specialized extracellular matrix structures that provide structural support and regulate cellular behavior. Although BM composition and organization are dynamically remodeled during development and tissue regeneration, the remodeling of individual BM components in the cardiovascular system (CVS) remain incompletely characterized. Here, we focused on Collagen4 (Col4), a major structural component of BMs, to investigate its remodeling during cardiovascular development and regeneration. To monitor the remodeling of Col4 in vivo, the Venus fluorescent tag was integrated in frame into the zebrafish endogenous col4a1 and col4a2 genes, enabling live confocal imaging of expression. Our data reveal dynamic patterns of Col4a1 and Col4a2 within the developing CVS, with deposition progressively increasing as vascular and cardiac structures develop and mature during both development and regeneration. In the regenerating heart, Col4a2 accumulated at the injury site during early stages of regeneration and became increasingly associated with the newly regenerated myocardium. Together, these findings demonstrate that Col4 deposition is dynamically reorganized across developmental and regenerative stages of CVS and describe new tools for following Col4 dynamics in vivo.
SN - 2692-8205
ER -