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Peer-reviewedCC BY 4.0Chemistry (Weinheim an Der Bergstrasse, Germany)

Cellular Membrane‐Targeted Europium(III) Probe for Selective and Time‐Resolved Detection of Peroxynitrite

  • Jamie Webb1
  • Rebecca Tiffany1
  • Karolis Norvaisa2
  • Hennie Valkenier2
  • Lea Miebach3,4
  • Sander Bekeschus3,5
  • Stephen J. Butler1
5 affiliations
  1. 1Department of Chemistry Loughborough University Loughborough UK
  2. 2Engineering of Molecular Nanosystems, Ecole Polytechnique De Bruxelles Université Libre de Bruxelles (ULB) Brussels Belgium
  3. 3ZIK Plasmatis Leibniz Institute for Plasma Science and Technology (INP) Greifswald Germany
  4. 4Department of Hematology and Oncology Greifswald University Medical Center Greifswald Germany
  5. 5Department of Dermatology, Venerology, and Allergology Rostock University Medical Center Rostock Germany
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Abstract

Peroxynitrite (ONOO−) is an extremely short‐lived and highly reactive species whose biological effects depend on its local concentration at the site of formation. Consequently, detecting and quantifying ONOO− requires probes that combine high selectivity with well‐defined subcellular localization. The cell membrane is a particularly important yet underexplored interface, as it is the first barrier encountered by extracellularly generated ONOO− and determines how much of this species penetrates into the cell. Such membrane‐proximal ONOO− formation occurs during inflammatory processes and oxidative stress–based therapies, and is especially relevant in cold atmospheric plasma treatment, where complex RONS mixtures interact with the membrane before intracellular effects arise. Here, we report Eu.Chol, a luminescent europium(III) complex that integrates a phenylboronic acid ONOO− reactive motif with a cholesterol anchor to localize the probe within lipid bilayers. We demonstrate that Eu.Chol incorporates efficiently into liposomes and Jurkat cells, enabling selective, membrane‐localized ONOO− detection with minimal response to H2O2. These results establish a modular europium(III)‐based scaffold for targeted ONOO− sensing, enabling potential incorporation of organelle‐specific targeting groups to probe RONS dynamics at defined sub‐cellular sites.

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Cite

BibTeXRIS
BibTeX
@article{webb2026cellular,
  title = {{Cellular Membrane‐Targeted Europium(III) Probe for Selective and Time‐Resolved Detection of Peroxynitrite}},
  author = {Jamie Webb and Rebecca Tiffany and Karolis Norvaisa and Hennie Valkenier and Lea Miebach and Sander Bekeschus and Stephen J. Butler},
  journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
  year = {2026},
  month = jul,
  doi = {10.1002/chem.71463},
  url = {https://doi.org/10.1002/chem.71463},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Cellular Membrane‐Targeted Europium(III) Probe for Selective and Time‐Resolved Detection of Peroxynitrite
AU  - Jamie Webb
AU  - Rebecca Tiffany
AU  - Karolis Norvaisa
AU  - Hennie Valkenier
AU  - Lea Miebach
AU  - Sander Bekeschus
AU  - Stephen J. Butler
PY  - 2026
DA  - 2026/07/27
JO  - Chemistry (Weinheim an Der Bergstrasse, Germany)
DO  - 10.1002/chem.71463
UR  - https://doi.org/10.1002/chem.71463
AB  - Peroxynitrite (ONOO−) is an extremely short‐lived and highly reactive species whose biological effects depend on its local concentration at the site of formation. Consequently, detecting and quantifying ONOO− requires probes that combine high selectivity with well‐defined subcellular localization. The cell membrane is a particularly important yet underexplored interface, as it is the first barrier encountered by extracellularly generated ONOO− and determines how much of this species penetrates into the cell. Such membrane‐proximal ONOO− formation occurs during inflammatory processes and oxidative stress–based therapies, and is especially relevant in cold atmospheric plasma treatment, where complex RONS mixtures interact with the membrane before intracellular effects arise. Here, we report Eu.Chol, a luminescent europium(III) complex that integrates a phenylboronic acid ONOO− reactive motif with a cholesterol anchor to localize the probe within lipid bilayers. We demonstrate that Eu.Chol incorporates efficiently into liposomes and Jurkat cells, enabling selective, membrane‐localized ONOO− detection with minimal response to H2O2. These results establish a modular europium(III)‐based scaffold for targeted ONOO− sensing, enabling potential incorporation of organelle‐specific targeting groups to probe RONS dynamics at defined sub‐cellular sites.
SN  - 0947-6539
SN  - 1521-3765
ER  -