The interaction between UDP-glycosyltransferase and CIPK11 regulates low nitrogen response in tobacco
Abstract
Low-nitrogen (LN) stress limits tobacco yield and quality, identifying LN tolerance genes is essential for efficient green production. Our study systematically characterized NtLNRU (Low Nitrogen Response UGT) and verified its function in LN stress. Its transcripts were significantly induced within 3 h of LN supply and peaked at 12 h. Hydroponic assays ofNtLNRU overexpression (OE) lines showed that root length, root dry weight and root/shoot ratio increased by 101.6 %, 167.8% and 151.3%, respectively, relative to wild-type (WT) under LN stress. Total leaf nitrogen and nitratenitrogen rose by 18.4% and 39.0%, respectively. Transcriptome analysisof OE lines identified 1936 up-regulated and2992down-regulated genesunder LN stress. KEGG enrichment highlighted “phenylpropanoid biosynthesis”, “nitrogen metabolism” and “alanine, aspartate and glutamate metabolism”. Weighted gene co-expression network analysis suggested a CBL-interacting protein kinase 11(NtCIPK11), peroxidase 3 and stress enhanced protein 2 may interact with NtLNRU.Subsequent yeast two-hybrid and luciferase complementation experiments confirmed NtLNRUinteracted with NtCIPK11.We propose that NtLNRUinteracts with NtCIPK11 toregulateLN toleranceby promoting root development and up-regulating genes for nitrogen utilization in tobacco.The study provides a novel gene resource for breeding tobacco cultivars facingnitrogen deficiency stress.
Related
- Physiology-informed UAV multispectral estimation of tobacco leaf nitrogen nutrition index for variable nitrogen management
- Nitrogen form regulates ginsenoside biosynthesis in Panax ginseng adventitious roots: comparative transcriptomics of ammonium- and nitrate-mediated pathways
- A non-canonical redox family protein regulates bacterial interaction machineries
- Bombyx mori p38 MAPK Regulates Cell Cycle Progression and Silk Gland Endoreplication via Interaction with Cell Cycle Genes
- Genome-wide analysis of genetic modifiers of Crohn’s disease and ulcerative colitis risk in response to tobacco smoking
BibTeX
@article{yang2026interaction,
title = {{The interaction between UDP-glycosyltransferase and CIPK11 regulates low nitrogen response in tobacco}},
author = {Weili Yang and Haoyue Feng and Hui Luo and Xingyou Yang and Haoran Xie and Yifei Lu and Bi Ren and Liming Lu and Liqin Li},
journal = {Frontiers in Plant Science},
year = {2026},
month = nov,
doi = {10.3389/fpls.2026.1957192},
url = {https://www.frontiersin.org/articles/10.3389/fpls.2026.1957192},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - The interaction between UDP-glycosyltransferase and CIPK11 regulates low nitrogen response in tobacco
AU - Weili Yang
AU - Haoyue Feng
AU - Hui Luo
AU - Xingyou Yang
AU - Haoran Xie
AU - Yifei Lu
AU - Bi Ren
AU - Liming Lu
AU - Liqin Li
PY - 2026
DA - 2026/11/04
JO - Frontiers in Plant Science
DO - 10.3389/fpls.2026.1957192
UR - https://www.frontiersin.org/articles/10.3389/fpls.2026.1957192
AB - Low-nitrogen (LN) stress limits tobacco yield and quality, identifying LN tolerance genes is essential for efficient green production. Our study systematically characterized NtLNRU (Low Nitrogen Response UGT) and verified its function in LN stress. Its transcripts were significantly induced within 3 h of LN supply and peaked at 12 h. Hydroponic assays ofNtLNRU overexpression (OE) lines showed that root length, root dry weight and root/shoot ratio increased by 101.6 %, 167.8% and 151.3%, respectively, relative to wild-type (WT) under LN stress. Total leaf nitrogen and nitratenitrogen rose by 18.4% and 39.0%, respectively. Transcriptome analysisof OE lines identified 1936 up-regulated and2992down-regulated genesunder LN stress. KEGG enrichment highlighted “phenylpropanoid biosynthesis”, “nitrogen metabolism” and “alanine, aspartate and glutamate metabolism”. Weighted gene co-expression network analysis suggested a CBL-interacting protein kinase 11(NtCIPK11), peroxidase 3 and stress enhanced protein 2 may interact with NtLNRU.Subsequent yeast two-hybrid and luciferase complementation experiments confirmed NtLNRUinteracted with NtCIPK11.We propose that NtLNRUinteracts with NtCIPK11 toregulateLN toleranceby promoting root development and up-regulating genes for nitrogen utilization in tobacco.The study provides a novel gene resource for breeding tobacco cultivars facingnitrogen deficiency stress.
SN - 1664-462X
ER -