A Dual Mechanism-Based Physiologically Based Pharmacokinetic Model for Predicting Mitoxantrone and Liposomal Mitoxantrone Disposition: Preclinical Validation in Rats and Human Extrapolation with Clinical Evaluation.
- 1Department of Clinical Pharmacology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, People's Republic of China.
Abstract
Purpose
This study aimed to develop a physiologically based pharmacokinetic (PBPK) model to quantitatively predict plasma and tissue concentrations of both liposomal-encapsulated and free mitoxantrone in rats and further extrapolate the model to human clinical scenarios.Methods
A dual-compartment PBPK framework was constructed to separately describe the disposition of liposomal carriers and released free mitoxantrone. Global sensitivity analysis (GSA) based on partial rank correlation coefficient (PRCC) was performed to identify key parameters. The external validation was evaluated using rat plasma pharmacokinetic and human clinical data from previous reported studies. Allometric scaling was applied to extrapolate the model to adult humans, and Monte Carlo simulation was utilized to predict clinical plasma concentrations under fixed clinical doses of 20, 30, and 40 mg in lymphoma patients.Results
PRCC analysis results demonstrated that tissue DNA binding capacity (T_DNA) and tissue-to-plasma liposome partition coefficient (Kp_n) were the most dominant parameters governing free drug and liposomal exposure, respectively (PRCC > 0.9). Overall average absolute fold error (AAFE) for tissue predictions was below 2.0. A slightly higher AAFE of 2.16 was observed for free drug submodel, which still complied with the widely accepted 3-fold error threshold for PBPK validation. Model external validation results also demonstrated acceptable predictive capacity of the model for both total and free mitoxantrone concentrations across preclinical and clinical dose levels (AAFE < 3). For human extrapolation, over 92% of clinically observed plasma concentrations from 37 lymphoma patients fell within the model's 90% predictive interval.Conclusion
This study established a robust, mechanism-driven PBPK model that distinguished encapsulated and free mitoxantrone disposition. The model achieved reliable prediction performance and effectively interprets pharmacokinetic characteristics of PEGylated liposomal mitoxantrone. The present framework provided a valuable mechanistic reference for the construction and optimization of liposomal mitoxantrone PBPK models and supported future translational pharmacokinetic studies of liposomal formulations.
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BibTeX
@article{xu2026dual,
title = {{A Dual Mechanism-Based Physiologically Based Pharmacokinetic Model for Predicting Mitoxantrone and Liposomal Mitoxantrone Disposition: Preclinical Validation in Rats and Human Extrapolation with Clinical Evaluation.}},
author = {Yuxiang Xu and Shuo Li and Zhixuan Yang and Yushuo Qiu and Jiameng Dong and Xiaonan Gao and Jinglin Gao and Zhangying Feng and Mingxia Wang},
journal = {International journal of nanomedicine},
year = {2026},
month = oct,
doi = {10.2147/ijn.s638932},
url = {https://europepmc.org/article/MED/42974179},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - A Dual Mechanism-Based Physiologically Based Pharmacokinetic Model for Predicting Mitoxantrone and Liposomal Mitoxantrone Disposition: Preclinical Validation in Rats and Human Extrapolation with Clinical Evaluation.
AU - Yuxiang Xu
AU - Shuo Li
AU - Zhixuan Yang
AU - Yushuo Qiu
AU - Jiameng Dong
AU - Xiaonan Gao
AU - Jinglin Gao
AU - Zhangying Feng
AU - Mingxia Wang
PY - 2026
DA - 2026/10/29
JO - International journal of nanomedicine
DO - 10.2147/ijn.s638932
UR - https://europepmc.org/article/MED/42974179
AB - Purpose This study aimed to develop a physiologically based pharmacokinetic (PBPK) model to quantitatively predict plasma and tissue concentrations of both liposomal-encapsulated and free mitoxantrone in rats and further extrapolate the model to human clinical scenarios.Methods A dual-compartment PBPK framework was constructed to separately describe the disposition of liposomal carriers and released free mitoxantrone. Global sensitivity analysis (GSA) based on partial rank correlation coefficient (PRCC) was performed to identify key parameters. The external validation was evaluated using rat plasma pharmacokinetic and human clinical data from previous reported studies. Allometric scaling was applied to extrapolate the model to adult humans, and Monte Carlo simulation was utilized to predict clinical plasma concentrations under fixed clinical doses of 20, 30, and 40 mg in lymphoma patients.Results PRCC analysis results demonstrated that tissue DNA binding capacity (T_DNA) and tissue-to-plasma liposome partition coefficient (Kp_n) were the most dominant parameters governing free drug and liposomal exposure, respectively (PRCC > 0.9). Overall average absolute fold error (AAFE) for tissue predictions was below 2.0. A slightly higher AAFE of 2.16 was observed for free drug submodel, which still complied with the widely accepted 3-fold error threshold for PBPK validation. Model external validation results also demonstrated acceptable predictive capacity of the model for both total and free mitoxantrone concentrations across preclinical and clinical dose levels (AAFE < 3). For human extrapolation, over 92% of clinically observed plasma concentrations from 37 lymphoma patients fell within the model's 90% predictive interval.Conclusion This study established a robust, mechanism-driven PBPK model that distinguished encapsulated and free mitoxantrone disposition. The model achieved reliable prediction performance and effectively interprets pharmacokinetic characteristics of PEGylated liposomal mitoxantrone. The present framework provided a valuable mechanistic reference for the construction and optimization of liposomal mitoxantrone PBPK models and supported future translational pharmacokinetic studies of liposomal formulations.
SN - 1176-9114
SN - 1178-2013
ER -