Different Analytical Modes on Automatic Hematology Analyzers for Testing Quality Control Materials and Commutability Assessment
- 1Department of Clinical Laboratory Wuhan Center for Clinical Laboratory Wuhan China
- 2Center for Clinical Laboratory General Hospital of the Yangtze River Shipping Wuhan Brain Hospital Wuhan China
Abstract
Introduction: Hematological disorders, critical for diagnosis, and monitoring, rely on accurate complete blood count results from automated analyzers, the reliability of which depends on robust quality control (QC). However, the ability of commutability of commercial hematology QC materials (HQCM) to behave like patient samples across different systems, and the impact of analytical modes on results are not fully characterized. This study aimed to evaluate the analytical performance and commutability of two HQCM brands (Bioyuan and XC) across two hematology analyzers (Sysmex XN‐1000 and Mindray BC‐6800 Plus).
Methods: We systematically compared results between QC and sample measurement modes, between manual and autoloader sampling modes, and assessed commutability using clinical samples as a reference.
Results: The differences between QC and sample modes were analyzer and parameter specific, with WBC, HCT, and MCV most affected on the Sysmex system. Manual versus autoloader sampling on the Mindray analyzer introduced significant differences for most parameters across concentrations. Commutability assessment revealed that neither HQCM was universally commutable for all four directly measured parameters (WBC, RBC, HGB, PLT); noncommutability was frequent, particularly, at extreme concentrations, indicating significant matrix effects.
Conclusion: This study highlights that analytical (sampling mode and measurement mode) variables, along with material‐dependent matrix effects, can substantially influence QC results and their interpretation. Laboratories must standardize protocols and critically select QC materials to ensure reliable hematology testing and effective quality assurance. Future work should focus on developing more commutable reference materials and validating these findings in multicenter clinical settings.
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BibTeX
@article{ren2026different,
title = {{Different Analytical Modes on Automatic Hematology Analyzers for Testing Quality Control Materials and Commutability Assessment}},
author = {Bingshuang Ren and Ya Cheng and Hongping Ba and Weijia Cheng and Xuwei Zhou and Hui Zhen and Shimin Wu and Jie Gong},
journal = {International Journal of Laboratory Hematology},
year = {2026},
month = jul,
doi = {10.1111/ijlh.70179},
url = {https://doi.org/10.1111/ijlh.70179},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Different Analytical Modes on Automatic Hematology Analyzers for Testing Quality Control Materials and Commutability Assessment
AU - Bingshuang Ren
AU - Ya Cheng
AU - Hongping Ba
AU - Weijia Cheng
AU - Xuwei Zhou
AU - Hui Zhen
AU - Shimin Wu
AU - Jie Gong
PY - 2026
DA - 2026/07/22
JO - International Journal of Laboratory Hematology
DO - 10.1111/ijlh.70179
UR - https://doi.org/10.1111/ijlh.70179
AB - Introduction: Hematological disorders, critical for diagnosis, and monitoring, rely on accurate complete blood count results from automated analyzers, the reliability of which depends on robust quality control (QC). However, the ability of commutability of commercial hematology QC materials (HQCM) to behave like patient samples across different systems, and the impact of analytical modes on results are not fully characterized. This study aimed to evaluate the analytical performance and commutability of two HQCM brands (Bioyuan and XC) across two hematology analyzers (Sysmex XN‐1000 and Mindray BC‐6800 Plus). Methods: We systematically compared results between QC and sample measurement modes, between manual and autoloader sampling modes, and assessed commutability using clinical samples as a reference. Results: The differences between QC and sample modes were analyzer and parameter specific, with WBC, HCT, and MCV most affected on the Sysmex system. Manual versus autoloader sampling on the Mindray analyzer introduced significant differences for most parameters across concentrations. Commutability assessment revealed that neither HQCM was universally commutable for all four directly measured parameters (WBC, RBC, HGB, PLT); noncommutability was frequent, particularly, at extreme concentrations, indicating significant matrix effects. Conclusion: This study highlights that analytical (sampling mode and measurement mode) variables, along with material‐dependent matrix effects, can substantially influence QC results and their interpretation. Laboratories must standardize protocols and critically select QC materials to ensure reliable hematology testing and effective quality assurance. Future work should focus on developing more commutable reference materials and validating these findings in multicenter clinical settings.
SN - 1751-5521
SN - 1751-553X
ER -