LNK expression, subcellular localization and survival associations in breast, cervical and colorectal cancer
Abstract
The role of lymphocyte adaptor protein (LNK) in breast, cervical and colorectal cancer remains incompletely characterized. We examined LNK expression, subcellular localization and associations with survival across these cancers. Gene Expression Profiling Interactive Analysis and cBioPortal were used to assess LNK messenger ribonucleic acid (mRNA) expression, while Kaplan-Meier Plotter was used for exploratory survival analyses. Western blotting and quantitative confocal immunofluorescence assessed protein expression and localization in 12 cancer cell lines and three non-tumoral controls. LNK mRNA expression varied among tumor subtypes and was significantly lower in cervical tumors than in adjacent normal tissue. Higher expression was associated with longer overall and recurrence-free survival in triple-negative breast cancer but shorter survival for both endpoints in colorectal cancer; no significant associations were observed in cervical cancer. LNK was detected in the nucleus and cytoplasm of all cancer cell lines. Significant nuclear predominance was observed in one of four triple-negative breast cancer cell lines and three of four cervical cancer cell lines, whereas their non-tumoral controls showed cytoplasmic predominance. Colorectal cancer cell lines displayed heterogeneous subcellular distributions. These exploratory findings suggest cancer type-specific associations between LNK expression and survival that require independent validation, while nuclear localization warrants investigation of potential nuclear functions.
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BibTeX
@article{mantillaollarves2026expression,
title = {{LNK expression, subcellular localization and survival associations in breast, cervical and colorectal cancer}},
author = {Jessica C Mantilla-Ollarves and Alejandro Sánchez-Carballido and Alejandro Ordaz-Ramos and Cecilia Aguilar and Miguel Tapia-Rodríguez and Liliana Cortés-Ballinas and Laura Velázquez and Leticia Rocha-Zavaleta},
journal = {Biomolecules and Biomedicine},
year = {2026},
month = sep,
doi = {10.17305/bb.2026.14287},
url = {https://hal.science/hal-05837578v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - LNK expression, subcellular localization and survival associations in breast, cervical and colorectal cancer
AU - Jessica C Mantilla-Ollarves
AU - Alejandro Sánchez-Carballido
AU - Alejandro Ordaz-Ramos
AU - Cecilia Aguilar
AU - Miguel Tapia-Rodríguez
AU - Liliana Cortés-Ballinas
AU - Laura Velázquez
AU - Leticia Rocha-Zavaleta
PY - 2026
DA - 2026/09/17
JO - Biomolecules and Biomedicine
DO - 10.17305/bb.2026.14287
UR - https://hal.science/hal-05837578v1
AB - The role of lymphocyte adaptor protein (LNK) in breast, cervical and colorectal cancer remains incompletely characterized. We examined LNK expression, subcellular localization and associations with survival across these cancers. Gene Expression Profiling Interactive Analysis and cBioPortal were used to assess LNK messenger ribonucleic acid (mRNA) expression, while Kaplan-Meier Plotter was used for exploratory survival analyses. Western blotting and quantitative confocal immunofluorescence assessed protein expression and localization in 12 cancer cell lines and three non-tumoral controls. LNK mRNA expression varied among tumor subtypes and was significantly lower in cervical tumors than in adjacent normal tissue. Higher expression was associated with longer overall and recurrence-free survival in triple-negative breast cancer but shorter survival for both endpoints in colorectal cancer; no significant associations were observed in cervical cancer. LNK was detected in the nucleus and cytoplasm of all cancer cell lines. Significant nuclear predominance was observed in one of four triple-negative breast cancer cell lines and three of four cervical cancer cell lines, whereas their non-tumoral controls showed cytoplasmic predominance. Colorectal cancer cell lines displayed heterogeneous subcellular distributions. These exploratory findings suggest cancer type-specific associations between LNK expression and survival that require independent validation, while nuclear localization warrants investigation of potential nuclear functions.
SN - 2831-0896
SN - 2831-090X
ER -