Mouse phospholipid phosphatase 6 regulates dendritic cell cholesterol, macropinocytosis, and allergen sensitization
Abstract
Lipid phosphate phosphatases are a family of enzymes with diverse cellular metabolic functions. Phospholipid phosphatase 6 (PLPP6) is a regulator of cellular polyisoprenyl phosphates; however, its in vivo functions remain to be determined. Here, mouse PLPP6 was characterized to possess similar catalytic properties as the human enzyme. Plpp6 knockout mice ( Plpp6 −/− ) were generated and displayed decreased airway allergen sensitization, pointing to a role for PLPP6 in the early events of lung allergic responses. Dendritic cell (DC) responses were investigated and endocytosis of allergen via macropinocytosis was decreased in Plpp6 −/− DCs that had lower cholesterol content. When reversed by cholesterol loading, the DC macropinocytosis defect is corrected. Adoptive transfer of Plpp6 −/− DCs to wild-type mice during sensitization was sufficient to decrease allergen-induced responses . Together, our findings have identified PLPP6 as a pivotal regulator of DC cholesterol content and macropinocytosis, cellular mechanisms that are important for pathologic responses in allergen-induced lung inflammation.
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BibTeX
@article{bruggemann2026mouse,
title = {{Mouse phospholipid phosphatase 6 regulates dendritic cell cholesterol, macropinocytosis, and allergen sensitization}},
author = {Thayse Brüggemann and Troy Carlo and Nandini Krishnamoorthy and Melody Duvall and Raja-Elie Abdulnour and Julie Nijmeh and Hong Yong Peh and Harilaos Filippakis and Roxanne Croze and Byoungsook Goh and Sungwhan Oh and Bruce Levy},
journal = {iScience},
year = {2026},
month = oct,
doi = {10.1016/j.isci.2022.105185},
url = {https://hal.science/hal-05787787v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Mouse phospholipid phosphatase 6 regulates dendritic cell cholesterol, macropinocytosis, and allergen sensitization
AU - Thayse Brüggemann
AU - Troy Carlo
AU - Nandini Krishnamoorthy
AU - Melody Duvall
AU - Raja-Elie Abdulnour
AU - Julie Nijmeh
AU - Hong Yong Peh
AU - Harilaos Filippakis
AU - Roxanne Croze
AU - Byoungsook Goh
AU - Sungwhan Oh
AU - Bruce Levy
PY - 2026
DA - 2026/10/09
JO - iScience
DO - 10.1016/j.isci.2022.105185
UR - https://hal.science/hal-05787787v1
AB - Lipid phosphate phosphatases are a family of enzymes with diverse cellular metabolic functions. Phospholipid phosphatase 6 (PLPP6) is a regulator of cellular polyisoprenyl phosphates; however, its in vivo functions remain to be determined. Here, mouse PLPP6 was characterized to possess similar catalytic properties as the human enzyme. Plpp6 knockout mice ( Plpp6 −/− ) were generated and displayed decreased airway allergen sensitization, pointing to a role for PLPP6 in the early events of lung allergic responses. Dendritic cell (DC) responses were investigated and endocytosis of allergen via macropinocytosis was decreased in Plpp6 −/− DCs that had lower cholesterol content. When reversed by cholesterol loading, the DC macropinocytosis defect is corrected. Adoptive transfer of Plpp6 −/− DCs to wild-type mice during sensitization was sufficient to decrease allergen-induced responses . Together, our findings have identified PLPP6 as a pivotal regulator of DC cholesterol content and macropinocytosis, cellular mechanisms that are important for pathologic responses in allergen-induced lung inflammation.
SN - 2589-0042
ER -