A semi-automated genetic screen using genome-wide association analysis identifies modulators of stress-induced sleep in Caenorhabditis elegans
Abstract
Animals sleep more when they are sick. In Caenorhabditis elegans, stress-induced sleep (SIS) follows cellular injury such as exposure to ultraviolet (UV) light. The genetic regulators of SIS remain incompletely defined. Using robotic automation, high-throughput imaging, and genome-wide association analysis, we performed a semi-automated screen of 940 whole-genome-sequenced Million Mutation Project (MMP) strains. We quantified behavioral activity and quiescence before and after ultraviolet (UV) radiation. We applied the Sequence Kernel Association Test (SKAT) to this behavioral data to prioritize a set of associated genes and observed significant enrichment of known SIS genetic regulators. Based on these results, we conducted a candidate validation screen for additional genes regulating SIS. We identified three genes (strd-1, cla-1, and egl-8), mutations in which reproducibly influence SIS. Further exploration of these genes holds potential for enhancing our understanding of the molecular basis of SIS. These findings establish a pipeline for automated behavioral phenotyping coupled with gene-based association to accelerate studies of C. elegans neurogenetics.
Versions
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BibTeX
@article{li2026semiautomated,
title = {{A semi-automated genetic screen using genome-wide association analysis identifies modulators of stress-induced sleep in Caenorhabditis elegans}},
author = {Zihao (John) Li and Helya Honarpisheh and Sreekar Kutagulla and Kerry M LeCure and Jing Liang and David M Raizen and Christopher Fang-Yen},
journal = {G3: Genes | Genomes | Genetics},
year = {2026},
month = sep,
doi = {10.1093/g3journal/jkag266},
url = {https://doi.org/10.1093/g3journal/jkag266},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - A semi-automated genetic screen using genome-wide association analysis identifies modulators of stress-induced sleep in Caenorhabditis elegans
AU - Zihao (John) Li
AU - Helya Honarpisheh
AU - Sreekar Kutagulla
AU - Kerry M LeCure
AU - Jing Liang
AU - David M Raizen
AU - Christopher Fang-Yen
PY - 2026
DA - 2026/09/28
JO - G3: Genes | Genomes | Genetics
DO - 10.1093/g3journal/jkag266
UR - https://doi.org/10.1093/g3journal/jkag266
AB - Animals sleep more when they are sick. In Caenorhabditis elegans, stress-induced sleep (SIS) follows cellular injury such as exposure to ultraviolet (UV) light. The genetic regulators of SIS remain incompletely defined. Using robotic automation, high-throughput imaging, and genome-wide association analysis, we performed a semi-automated screen of 940 whole-genome-sequenced Million Mutation Project (MMP) strains. We quantified behavioral activity and quiescence before and after ultraviolet (UV) radiation. We applied the Sequence Kernel Association Test (SKAT) to this behavioral data to prioritize a set of associated genes and observed significant enrichment of known SIS genetic regulators. Based on these results, we conducted a candidate validation screen for additional genes regulating SIS. We identified three genes (strd-1, cla-1, and egl-8), mutations in which reproducibly influence SIS. Further exploration of these genes holds potential for enhancing our understanding of the molecular basis of SIS. These findings establish a pipeline for automated behavioral phenotyping coupled with gene-based association to accelerate studies of C. elegans neurogenetics.
SN - 2160-1836
ER -