Peer-reviewedCC BY-NC-NDSTAR protocols
Protocol for confocal microscopy-based localization of chorismate mutase protein of Mycobacterium tuberculosis in trans-Golgi compartment.
- 1Laboratory of Molecular Cell Biology, BRIC-Centre for DNA Fingerprinting and Diagnostics (CDFD), Inner Ring Road, Uppal, Hyderabad 500039, Telangana, India; Graduate Studies, Regional Centre for Biotechnology, Faridabad 121001, Haryana (NCR Delhi), India.
- 2Laboratory of Molecular Cell Biology, BRIC-Centre for DNA Fingerprinting and Diagnostics (CDFD), Inner Ring Road, Uppal, Hyderabad 500039, Telangana, India. Electronic address: [email protected].
Abstract
Here, we present a confocal microscopy protocol to determine the localization of Mycobacterium tuberculosis chorismate mutase (MtbCM) in trans-Golgi compartment. We co-transfect HEK293 cells with either pEGFP-C1-MtbCM and mCherry-TGOLN2 (trans-Golgi marker) or pEGFP-C1 and mCherry-TGOLN2 as a control, using Lipofectamine 2000. We describe steps for constructing pEGFP-C1-MtbCM and performing co-transfection and image acquisition using confocal microscopy. This protocol provides a representative procedure to determine the subcellular localization of the mycobacterial protein to the trans-Golgi compartment. For complete details on the use and execution of this protocol, please refer to Pal et al.¹.
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BibTeX
@article{maurya2026protocol,
title = {{Protocol for confocal microscopy-based localization of chorismate mutase protein of Mycobacterium tuberculosis in trans-Golgi compartment.}},
author = {Vandana Maurya and Sangita Mukhopadhyay},
journal = {STAR protocols},
year = {2026},
month = oct,
doi = {10.1016/j.xpro.2026.104859},
url = {https://europepmc.org/article/MED/42837261},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Protocol for confocal microscopy-based localization of chorismate mutase protein of Mycobacterium tuberculosis in trans-Golgi compartment.
AU - Vandana Maurya
AU - Sangita Mukhopadhyay
PY - 2026
DA - 2026/10/05
JO - STAR protocols
DO - 10.1016/j.xpro.2026.104859
UR - https://europepmc.org/article/MED/42837261
AB - Here, we present a confocal microscopy protocol to determine the localization of Mycobacterium tuberculosis chorismate mutase (MtbCM) in trans-Golgi compartment. We co-transfect HEK293 cells with either pEGFP-C1-MtbCM and mCherry-TGOLN2 (trans-Golgi marker) or pEGFP-C1 and mCherry-TGOLN2 as a control, using Lipofectamine 2000. We describe steps for constructing pEGFP-C1-MtbCM and performing co-transfection and image acquisition using confocal microscopy. This protocol provides a representative procedure to determine the subcellular localization of the mycobacterial protein to the trans-Golgi compartment. For complete details on the use and execution of this protocol, please refer to Pal et al.¹.
SN - 2666-1667
ER -