SNP analysis of sweet pumpkin to corroborate carotenoid accumulation using different inbred lines
- 1Division of Horticultural Biotechnology, Hankyong National University
- 2Breeding Solution Co., LTD.
- 3East West Seed International
Abstract
The differential fruit color of the sweet pumpkin (Cucurbita maxima) is due to the presence of variant levels of carotenoids. Apart from appealing culinary fruit characteristics, carotenoid composition is responsible for high nutritional values of sweet pumpkin. Single nucleotide polymorphism-based DNA markers is widely used in agronomic crops to understand the genetic architecture, physiology of the trait, and selection of cultivars. In this study, we analyzed various distinguishable SNP markers in CmaCh06G005910, CmaCh14G015700, CmaCh15G000800, CmaCh08G002870, CmaCh04G017950, and CmaCh17G014010 genes using HRM techniques in small, medium, and large sized pumpkin lines cultivated in South Korea. These genes are closely related to carotenoid biosynthesis pathway in sweet pumpkin. Mesocarp of the selected pumpkin were also subjected to HPLC quantification for α-carotene, β-carotene, β-cryptoxanthin, and zeaxanthin content. The genetic profiling and HPLC analysis revealed a pleotropic effect of carotenoid related genes. As an additional analysis, SNP markers in the CmaCh08G000410 gene previously reported to be associated with fruit length were evaluated because the study population included cultivars with diverse fruit sizes. The fruit length-linked marker produced distinguishable HRM peaks among lines exhibiting different fruit lengths. This genotype data will be aided as an exploratory data on understanding the carotenoid genes in the Korean cultivars. Furthermore, it helps the breeder to select the genetically informed nutrient enhanced cultivars.
Related
- Red–Blue and White Supplementary Light Increase Carotenoid Accumulation and Improve Fruit Quality in Satsuma Mandarin: Associations with Chlorophyll Content and Carotenogenic Gene Expression
- Induction of Carotenoid Accumulation by Salt Stress in a Newly Isolated Dysmorphococcus Strain from Antalya, Türkiye
- Cold acclimation of tomato plants is associated with differential expression of genes of the SlLHC family, carotenoid metabolism, and chlorophyll accumulation/degradation.
- Multi-omics reveals accumulation mechanisms underlying differences in sesquiterpenoid content across different growth environments of Atractylodes macrocephala rhizome
- The Effects of Different Companion Fungi on the Growth and Development of Cremastra appendiculata and the Accumulation of Bioactive Components in Its Pseudobulbs
BibTeX
@article{park2026analysis,
title = {{SNP analysis of sweet pumpkin to corroborate carotenoid accumulation using different inbred lines}},
author = {Jong-Geun Park and Song Samnang and Phillip Choe and Kwon-Kyoo Kang and Ill-Sup Nou},
journal = {Scientific Reports},
year = {2026},
month = jul,
doi = {10.1038/s41598-026-61479-6},
url = {https://doi.org/10.1038/s41598-026-61479-6},
note = {CC BY},
}
RIS
TY - JOUR
TI - SNP analysis of sweet pumpkin to corroborate carotenoid accumulation using different inbred lines
AU - Jong-Geun Park
AU - Song Samnang
AU - Phillip Choe
AU - Kwon-Kyoo Kang
AU - Ill-Sup Nou
PY - 2026
DA - 2026/07/01
JO - Scientific Reports
DO - 10.1038/s41598-026-61479-6
UR - https://doi.org/10.1038/s41598-026-61479-6
AB - The differential fruit color of the sweet pumpkin (Cucurbita maxima) is due to the presence of variant levels of carotenoids. Apart from appealing culinary fruit characteristics, carotenoid composition is responsible for high nutritional values of sweet pumpkin. Single nucleotide polymorphism-based DNA markers is widely used in agronomic crops to understand the genetic architecture, physiology of the trait, and selection of cultivars. In this study, we analyzed various distinguishable SNP markers in CmaCh06G005910, CmaCh14G015700, CmaCh15G000800, CmaCh08G002870, CmaCh04G017950, and CmaCh17G014010 genes using HRM techniques in small, medium, and large sized pumpkin lines cultivated in South Korea. These genes are closely related to carotenoid biosynthesis pathway in sweet pumpkin. Mesocarp of the selected pumpkin were also subjected to HPLC quantification for α-carotene, β-carotene, β-cryptoxanthin, and zeaxanthin content. The genetic profiling and HPLC analysis revealed a pleotropic effect of carotenoid related genes. As an additional analysis, SNP markers in the CmaCh08G000410 gene previously reported to be associated with fruit length were evaluated because the study population included cultivars with diverse fruit sizes. The fruit length-linked marker produced distinguishable HRM peaks among lines exhibiting different fruit lengths. This genotype data will be aided as an exploratory data on understanding the carotenoid genes in the Korean cultivars. Furthermore, it helps the breeder to select the genetically informed nutrient enhanced cultivars.
SN - 2045-2322
ER -