Positional cues, not Notch, direct neuroblast selection during early neurogenesis in the Drosophila embryo
Abstract
Notch-mediated lateral inhibition is a conserved patterning process that controls alternative cell fate decisions and produces regular cell fate patterns. Prevailing models posit that lateral inhibition singles out cells from fields of initially equipotent cells by amplifying stochastic fluctuations of Notch or pre-existing fate biases. Here, we revisited the role of Notch in early Drosophila neurogenesis, studying the dynamics of neuroblast specification by live imaging the transcription of two proneural genes, scute and lethal of scute. We found that proneural gene expression is biased spatially along the dorsal-ventral axis prior to germ band extension and that early proneural expression predicts neuroblast fate acquisition. This indicated that neuroblast specification is pre-patterned by positional cues. Additionally, positional cues appeared to instruct individual cells to delaminate in a correct stereotyped pattern in proneural mutant embryos. Finally, contrary to current models, Notch signaling, measured by E(spl)m8 expression, was not detectable within proneural clusters until after neuroblasts had initiated delamination. This indicated that Notch functions to stabilize rather than initiate fate decisions. We therefore propose that positional cues, not Notch, single out neuroblasts during early Drosophila neurogenesis, challenging long-held assumptions about the role of Notch in neuroblast selection.
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BibTeX
@article{green2026positional,
title = {{Positional cues, not Notch, direct neuroblast selection during early neurogenesis in the Drosophila embryo}},
author = {David Green and Khalil Mazouni and Marcel Nos and Francois Schweisguth},
journal = {Current Biology},
year = {2026},
month = sep,
doi = {10.1016/j.cub.2026.07.043},
url = {https://pasteur.hal.science/pasteur-05760683v1},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Positional cues, not Notch, direct neuroblast selection during early neurogenesis in the Drosophila embryo
AU - David Green
AU - Khalil Mazouni
AU - Marcel Nos
AU - Francois Schweisguth
PY - 2026
DA - 2026/09/07
JO - Current Biology
DO - 10.1016/j.cub.2026.07.043
UR - https://pasteur.hal.science/pasteur-05760683v1
AB - Notch-mediated lateral inhibition is a conserved patterning process that controls alternative cell fate decisions and produces regular cell fate patterns. Prevailing models posit that lateral inhibition singles out cells from fields of initially equipotent cells by amplifying stochastic fluctuations of Notch or pre-existing fate biases. Here, we revisited the role of Notch in early Drosophila neurogenesis, studying the dynamics of neuroblast specification by live imaging the transcription of two proneural genes, scute and lethal of scute. We found that proneural gene expression is biased spatially along the dorsal-ventral axis prior to germ band extension and that early proneural expression predicts neuroblast fate acquisition. This indicated that neuroblast specification is pre-patterned by positional cues. Additionally, positional cues appeared to instruct individual cells to delaminate in a correct stereotyped pattern in proneural mutant embryos. Finally, contrary to current models, Notch signaling, measured by E(spl)m8 expression, was not detectable within proneural clusters until after neuroblasts had initiated delamination. This indicated that Notch functions to stabilize rather than initiate fate decisions. We therefore propose that positional cues, not Notch, single out neuroblasts during early Drosophila neurogenesis, challenging long-held assumptions about the role of Notch in neuroblast selection.
SN - 0960-9822
SN - 1879-0445
ER -