Detection of Antipoly(ethylene glycol) IgM in Immune Serum by Oligo(ethylene glycol)-Conjugated Cello-oligosaccharide Assemblies
- 1Institute of Science Tokyo , , 2-12-1-H-121 Ookayama , Meguro-ku, ,
- 2Juntendo University , , 6-8-1 Hinode , , ,
- 3the Jikei University School of Medicine , , 163-1 Kashiwa-shita , , ,
Abstract
Adsorbing antibodies of interest onto solid supports displaying corresponding antigens is an efficient strategy for their subsequent purification and detection. We have previously demonstrated the potential of water-dispersible, antibiofouling, and designable cello-oligosaccharide assemblies for antibody detection via enzyme-linked immunosorbent assay (ELISA). However, whether antigen-conjugated cello-oligosaccharide assemblies can detect specific antibodies contained in immune serum remains unexplored. In this study, we demonstrate the utility of oligo(ethylene glycol) (OEG)-conjugated cello-oligosaccharide assemblies for detecting antipoly(ethylene glycol) (PEG) immunoglobulin M (IgM) in serum from immunized animals. Mouse sera containing anti-PEG IgMs, induced by immunogenic polymers, were utilized for evaluation. ELISA results revealed that anti-PEG IgMs in the serum with no heat-inactivation or depletion were successfully detected by cello-oligosaccharide assemblies displaying OEG chains with at least three repeating ethylene glycol units. ELISAs using a series of cello-oligosaccharide assemblies displaying OEG chains indicated that the conjugation rate of OEG chains influences anti-PEG IgM binding. Furthermore, increasing the serum concentration led to enhanced detection efficacy for anti-PEG IgM, likely due to the superior antibiofouling properties of the cello-oligosaccharide assemblies, which minimize nonspecific adsorption of serum proteins. The concentration of the assemblies also significantly influenced the detection efficacy. Additionally, 7 days after a single intravenous injection of OEG-conjugated cello-oligosaccharide assemblies without micrometer-scale aggregates, ELISA signals for IgMs against PEG chains, OEG chains, and cello-oligosaccharide moieties were comparable to those of saline-treated controls. These findings demonstrated that OEG-conjugated cello-oligosaccharide assemblies hold considerable potential for the detection of anti-PEG IgMs in immune serum with no heat-inactivation or depletion.
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BibTeX
@article{sugiura2026detection,
title = {{Detection of Antipoly(ethylene glycol) IgM in Immune Serum by Oligo(ethylene glycol)-Conjugated Cello-oligosaccharide Assemblies}},
author = {Kai Sugiura and Yuuki Hata and Hiroshi Tanaka and Kouichi Shiraishi and Takeshi Serizawa},
journal = {ACS Omega},
year = {2026},
month = nov,
doi = {10.1021/acsomega.6c04925},
url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04925/5466299/Detection-of-Antipoly-ethylene-glycol-IgM-in},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Detection of Antipoly(ethylene glycol) IgM in Immune Serum by Oligo(ethylene glycol)-Conjugated Cello-oligosaccharide Assemblies
AU - Kai Sugiura
AU - Yuuki Hata
AU - Hiroshi Tanaka
AU - Kouichi Shiraishi
AU - Takeshi Serizawa
PY - 2026
DA - 2026/11/09
JO - ACS Omega
DO - 10.1021/acsomega.6c04925
UR - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04925/5466299/Detection-of-Antipoly-ethylene-glycol-IgM-in
AB - Adsorbing antibodies of interest onto solid supports displaying corresponding antigens is an efficient strategy for their subsequent purification and detection. We have previously demonstrated the potential of water-dispersible, antibiofouling, and designable cello-oligosaccharide assemblies for antibody detection via enzyme-linked immunosorbent assay (ELISA). However, whether antigen-conjugated cello-oligosaccharide assemblies can detect specific antibodies contained in immune serum remains unexplored. In this study, we demonstrate the utility of oligo(ethylene glycol) (OEG)-conjugated cello-oligosaccharide assemblies for detecting antipoly(ethylene glycol) (PEG) immunoglobulin M (IgM) in serum from immunized animals. Mouse sera containing anti-PEG IgMs, induced by immunogenic polymers, were utilized for evaluation. ELISA results revealed that anti-PEG IgMs in the serum with no heat-inactivation or depletion were successfully detected by cello-oligosaccharide assemblies displaying OEG chains with at least three repeating ethylene glycol units. ELISAs using a series of cello-oligosaccharide assemblies displaying OEG chains indicated that the conjugation rate of OEG chains influences anti-PEG IgM binding. Furthermore, increasing the serum concentration led to enhanced detection efficacy for anti-PEG IgM, likely due to the superior antibiofouling properties of the cello-oligosaccharide assemblies, which minimize nonspecific adsorption of serum proteins. The concentration of the assemblies also significantly influenced the detection efficacy. Additionally, 7 days after a single intravenous injection of OEG-conjugated cello-oligosaccharide assemblies without micrometer-scale aggregates, ELISA signals for IgMs against PEG chains, OEG chains, and cello-oligosaccharide moieties were comparable to those of saline-treated controls. These findings demonstrated that OEG-conjugated cello-oligosaccharide assemblies hold considerable potential for the detection of anti-PEG IgMs in immune serum with no heat-inactivation or depletion.
SN - 2470-1343
ER -