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PreprintCC BYbioRxiv

Integrative identification of Tetratostichococcus sp. CCAH0201 (Trebouxiophyceae, Chlorophyta) from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam

  1. 1Ho Chi Minh City University of Industry and Trade (HUIT),

Abstract

A microalgal strain from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam, was examined by light microscopy and three molecular markers. Thirty-six cells from 22 micrographs measured 6.68-12.74 by 3.49-4.79 micrometres. Homologous phylogenetic windows comprised 550 nucleotides for SSU rDNA, 711 for ITS and 1,170 for rbcL. CCAH0201, P1 and Tetratostichococcus jenerensis SAG 2138 shared the SSU 3-prime sequence, whereas ITS and rbcL placed CCAH0201 with P1 (SH-aLRT/UFBoot 87/94 and 96.9/99). CCAH0201 differed from P1 at 22/711 ITS and 4/1,170 rbcL sites. Structure-guided ITS2 comparison identified one strict CBC, one two-sided change from a wobble pair and three hemi-CBCs relative to P1. CCAH0201 is thus identified as Tetratostichococcus sp. in a lineage close to P1.

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BibTeX
@misc{nguyen2026integrative,
  title = {{Integrative identification of Tetratostichococcus sp. CCAH0201 (Trebouxiophyceae, Chlorophyta) from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam}},
  author = {T.-L. Nguyen and H.-D. Tran},
  howpublished = {bioRxiv},
  year = {2026},
  month = nov,
  doi = {10.64898/2026.11.05.764477},
  url = {https://www.biorxiv.org/content/10.64898/2026.11.05.764477v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - Integrative identification of Tetratostichococcus sp. CCAH0201 (Trebouxiophyceae, Chlorophyta) from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam
AU  - T.-L. Nguyen
AU  - H.-D. Tran
PY  - 2026
DA  - 2026/11/05
PB  - bioRxiv
DO  - 10.64898/2026.11.05.764477
UR  - https://www.biorxiv.org/content/10.64898/2026.11.05.764477v1
AB  - A microalgal strain from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam, was examined by light microscopy and three molecular markers. Thirty-six cells from 22 micrographs measured 6.68-12.74 by 3.49-4.79 micrometres. Homologous phylogenetic windows comprised 550 nucleotides for SSU rDNA, 711 for ITS and 1,170 for rbcL. CCAH0201, P1 and Tetratostichococcus jenerensis SAG 2138 shared the SSU 3-prime sequence, whereas ITS and rbcL placed CCAH0201 with P1 (SH-aLRT/UFBoot 87/94 and 96.9/99). CCAH0201 differed from P1 at 22/711 ITS and 4/1,170 rbcL sites. Structure-guided ITS2 comparison identified one strict CBC, one two-sided change from a wobble pair and three hemi-CBCs relative to P1. CCAH0201 is thus identified as Tetratostichococcus sp. in a lineage close to P1.
ER  -