Milk-derived casein glycomacropeptide improves colonic mucus function under Western-style diet feeding in a sialylation-dependent manner
Abstract
The colonic mucus layer is the primary interface between the host and the gut microbiota. It serves both as an ecological niche for bacteria and a barrier protecting the host from microbial exposure. Disruption of the mucus layer, particularly under Western-style diet (WSD) feeding, increases the risk of infection and inflammation. Here, we identify casein glycomacropeptide (CGMP), a milk-derived glycopeptide, as a novel dietary supplement capable of preserving mucus function under WSD consumption. Notably, we demonstrate that the sialylation level of CGMP is a key determinant of its protective effects. Supplementation of highly sialylated CGMP not only prevented WSD-induced mucus defects but also altered the gut microbiota composition, enhancing beneficial bacterial genera, particularly Bifidobacterium. Mechanistically, bacterial shifts were associated with increased production of the short-chain fatty acid propionate, which can induce mucus growth. Our findings thus reveal sialylated CGMP as a promising prebiotic supplement to counteract diet-induced mucus dysfunction, highlighting the importance of protein-bound glycan structures in modulating host-microbiota interaction.
Related
- Nfia-mediated troponin C activation enhances pluripotent stem cell-derived cardiomyocyte function and improves cardiac remodeling after myocardial infarction
- Magnetic Fields Improves the Cellular function in Hyperglycaemia
- GlycoStamp-seq: Single-cell nascent sialoglycometabolomics captures sialylation dynamics in immune cells of an oxaliplatin-treated bladder cancer mouse model
- Rapsyn overexpression improves neuromuscular junction and skeletal muscle function after tourniquet-induced mouse hindlimb ischemia-reperfusion injuries
- The gut microbiota-derived butyrate improves gestational glucose homeostasis through HDAC3 inhibition and epigenetic activation of the SCGN/GLP-1 axis.
BibTeX
@article{wongkuna2026milkderived,
title = {{Milk-derived casein glycomacropeptide improves colonic mucus function under Western-style diet feeding in a sialylation-dependent manner}},
author = {Supapit Wongkuna and Vishnu Prasoodanan P.K. and Sandra Holmberg and Ann Bjørnshave and Bjoern Schroeder},
journal = {Food Research International},
year = {2026},
month = sep,
doi = {10.1016/j.foodres.2025.117206},
url = {https://hal.science/hal-05772042v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - Milk-derived casein glycomacropeptide improves colonic mucus function under Western-style diet feeding in a sialylation-dependent manner
AU - Supapit Wongkuna
AU - Vishnu Prasoodanan P.K.
AU - Sandra Holmberg
AU - Ann Bjørnshave
AU - Bjoern Schroeder
PY - 2026
DA - 2026/09/30
JO - Food Research International
DO - 10.1016/j.foodres.2025.117206
UR - https://hal.science/hal-05772042v1
AB - The colonic mucus layer is the primary interface between the host and the gut microbiota. It serves both as an ecological niche for bacteria and a barrier protecting the host from microbial exposure. Disruption of the mucus layer, particularly under Western-style diet (WSD) feeding, increases the risk of infection and inflammation. Here, we identify casein glycomacropeptide (CGMP), a milk-derived glycopeptide, as a novel dietary supplement capable of preserving mucus function under WSD consumption. Notably, we demonstrate that the sialylation level of CGMP is a key determinant of its protective effects. Supplementation of highly sialylated CGMP not only prevented WSD-induced mucus defects but also altered the gut microbiota composition, enhancing beneficial bacterial genera, particularly Bifidobacterium. Mechanistically, bacterial shifts were associated with increased production of the short-chain fatty acid propionate, which can induce mucus growth. Our findings thus reveal sialylated CGMP as a promising prebiotic supplement to counteract diet-induced mucus dysfunction, highlighting the importance of protein-bound glycan structures in modulating host-microbiota interaction.
SN - 0963-9969
ER -