Comparative genomics of Rhodospirillum rubrum S1 lineages reveals significant differences from the reference genome.
- 1Department of Biology, Marburg University, Marburg, Germany.
- 2Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
- 3Microbes-for-Climate (M4C) Cluster of Excellence & SYNMIKRO, Marburg, Germany.
Abstract
Laboratory cultivation imposes selective pressures that drive rapid bacterial evolution, compromising experimental reproducibility. Here, comparative phenotypic and genomic analyses revealed significant divergence between two Rhodospirillum rubrum S1 lineages originally obtained from the same culture collection. Although both shared baseline variations relative to the reference genome, their trajectories diverged. A recently sourced line (DSM 467) exhibited few additional mutations, including a fliF frameshift abolishing motility. Conversely, a long-maintained laboratory line remained motile but harbored numerous unique variants, particularly within stress-responsive histidine kinases. These results demonstrate passaging-induced genomic drift, highlighting the necessity of cryopreservation and minimal serial cultivation to ensure strain fidelity.
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BibTeX
@article{pohl2026comparative,
title = {{Comparative genomics of Rhodospirillum rubrum S1 lineages reveals significant differences from the reference genome.}},
author = {Sebastian Pöhl and Anke Treuner-Lange and Martin Thanbichler},
journal = {MicroPublication biology},
year = {2026},
month = oct,
doi = {10.17912/micropub.biology.002395},
url = {https://europepmc.org/article/MED/42997190},
note = {CC BY},
}
RIS
TY - JOUR
TI - Comparative genomics of Rhodospirillum rubrum S1 lineages reveals significant differences from the reference genome.
AU - Sebastian Pöhl
AU - Anke Treuner-Lange
AU - Martin Thanbichler
PY - 2026
DA - 2026/10/20
JO - MicroPublication biology
DO - 10.17912/micropub.biology.002395
UR - https://europepmc.org/article/MED/42997190
AB - Laboratory cultivation imposes selective pressures that drive rapid bacterial evolution, compromising experimental reproducibility. Here, comparative phenotypic and genomic analyses revealed significant divergence between two Rhodospirillum rubrum S1 lineages originally obtained from the same culture collection. Although both shared baseline variations relative to the reference genome, their trajectories diverged. A recently sourced line (DSM 467) exhibited few additional mutations, including a fliF frameshift abolishing motility. Conversely, a long-maintained laboratory line remained motile but harbored numerous unique variants, particularly within stress-responsive histidine kinases. These results demonstrate passaging-induced genomic drift, highlighting the necessity of cryopreservation and minimal serial cultivation to ensure strain fidelity.
SN - 2578-9430
ER -