Cell cycle exit is required for differentiation and cell fate diversification but not neoblast specialisation in planarians
- 1Living Systems Institute, University of Exeter, Exeter, UK
Abstract
In planarians, adult stem cells constantly proliferate and differentiate into dozens of different cell types to maintain tissue homeostasis. However, how cell fate acquisition is coordinated with cell cycle progression remains poorly understood. To address this, we generated single-cell RNA-sequencing datasets from cells enriched for 2C or 4C DNA content and from animals subjected to RNAi against the cell cycle regulators cdh1 and h2b. We found that progenitor populations from multiple lineages contain actively cycling cells enriched in the 4C state. Cell fate acquisition to most cell types is acquired progressively during neoblast differentiation. Comparative analysis of cell cycle regulators confirmed a simplified regulatory repertoire in Schmidtea, supporting a central role for cdh1 in controlling cell cycle exit. RNAi-mediated knockdown of cdh1 caused homeostasis defects, impaired regeneration, accumulation of neoblasts and depletion of progenitors. Together, our findings demonstrate that cell cycle exit is required for terminal differentiation but not for neoblast specialisation. Instead, lineage commitment is initiated in actively cycling neoblasts, whereas terminal differentiation depends on cdh1-mediated cell cycle exit.
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BibTeX
@misc{peron2026cell,
title = {{Cell cycle exit is required for differentiation and cell fate diversification but not neoblast specialisation in planarians}},
author = {S. Peron and O.-M. Balta and A. Noronha and T. Smillie and V. Mason and A. Perez-Posada and J. Solana},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.16.750956},
url = {https://www.biorxiv.org/content/10.64898/2026.09.16.750956v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Cell cycle exit is required for differentiation and cell fate diversification but not neoblast specialisation in planarians
AU - S. Peron
AU - O.-M. Balta
AU - A. Noronha
AU - T. Smillie
AU - V. Mason
AU - A. Perez-Posada
AU - J. Solana
PY - 2026
DA - 2026/09/16
PB - bioRxiv
DO - 10.64898/2026.09.16.750956
UR - https://www.biorxiv.org/content/10.64898/2026.09.16.750956v1
AB - In planarians, adult stem cells constantly proliferate and differentiate into dozens of different cell types to maintain tissue homeostasis. However, how cell fate acquisition is coordinated with cell cycle progression remains poorly understood. To address this, we generated single-cell RNA-sequencing datasets from cells enriched for 2C or 4C DNA content and from animals subjected to RNAi against the cell cycle regulators cdh1 and h2b. We found that progenitor populations from multiple lineages contain actively cycling cells enriched in the 4C state. Cell fate acquisition to most cell types is acquired progressively during neoblast differentiation. Comparative analysis of cell cycle regulators confirmed a simplified regulatory repertoire in Schmidtea, supporting a central role for cdh1 in controlling cell cycle exit. RNAi-mediated knockdown of cdh1 caused homeostasis defects, impaired regeneration, accumulation of neoblasts and depletion of progenitors. Together, our findings demonstrate that cell cycle exit is required for terminal differentiation but not for neoblast specialisation. Instead, lineage commitment is initiated in actively cycling neoblasts, whereas terminal differentiation depends on cdh1-mediated cell cycle exit.
ER -