Nigella Sativa Oil versus Thyroxine in Carbimazole-Induced Hypothyroidism: Modulation of Oxidative Stress and Inflammatory Pathways
- 1Department of Anatomy & Embryology, Faculty of Medicine, Kasr Al-Ainy, Cairo University, Cairo, Egypt
- 2Department of Anatomy and Embryology, Faculty of Medicine, Kasr Al‑Ainy, Cairo University
- 3Department of Anatomy and Embryology, Faculty of Medicine, Kasr Al-Ainy, Cairo University
Abstract
Background: Reduction of thyroid hormone levels is known as hypothyroidism produces an alterable effect of bodily activities more willingly than a hormonal deficit. Aim of the Work: The present research was designed to compare the valuable influences of Nigella Sativa oil (NSO) and thyroxine on carbimazole-persuaded hypothyroidism. Materials and Methods: Forty rats were utilized and allocated into five groups. Group I (without treatment), included subgroups Ia and Ib were. Group II received distilled water, orally, included subgroups IIa and IIb. Group III was administered oral carbimazole, daily for three weeks (subgroup IIIa) and subgroup IIIb (recovered for an additional four weeks). All subgroups a and b were sacrificed after three and seven weeks, respectively. Group IV received carbimazole, like group III, followed by four weeks of daily oral NSO. Group V was given carbimazole like group III, followed by four weeks of oral thyroxine, daily. Serum levels of T3, T4, and TSH as well as oxidative markers in tissue; malondialdehyde and reduced glutathione were analyzed biochemically. In addition to histological examination, Caspase 3 and IL 6 immunohistochemical evaluations were performed. Results: Either NSO or thyroxine produced modifications effects in carbimazole-provoked histopathological changes of the thyroid gland, as demonstrated by a significant decrease in Caspase 3 immuno-expression, with more substantial regulating influence linked to thyroxine. In addition, both medications showed anti-inflammatory consequences (a decrease in immunohistochemical IL 6) and antioxidant capability (reduction in malondialdehyde and rise in reduced glutathione). Both NSO and thyroxine improved the thyroid function, nonetheless an additional impact related to thyroxine. Conclusion: Either NSO or thyroxine have an outstanding capability to minimize carbimazole- provoked negative consequences on the thyroid gland by regulation of oxidation-reduction state, as well as effective inhibition of inflammatory responses; thyroxine was more effective.
Related
- Fungal melanin as a bioactive biopolymer: structural diversity, oxidative stress modulation, anticancer mechanisms, and therapeutic perspectives.
- Gastroprotective effects of chlorella derived lutein via anti-inflammatory antioxidant and acid enzyme modulation
- MSC-derived extracellular vesicles for spinal cord injury: inflammatory microenvironment modulation, stage-dependent repair mechanisms, and translational challenges
- Exploring the therapeutic potential of Erycibe paniculata (Roxb.): metabolite profiling and antimalarial, anticancer, and modulation of NF-κB signaling pathway for anti-inflammatory activities
- Bioactivity-Guided Isolation of Cytoprotective Metabolites from Two Verbascum Species Reveals Differential Modulation of Cigarette Smoke-Induced Inflammation and Oxidative Stress
BibTeX
@article{sayed2026nigella,
title = {{Nigella Sativa Oil versus Thyroxine in Carbimazole-Induced Hypothyroidism: Modulation of Oxidative Stress and Inflammatory Pathways}},
author = {Walaa Sayed and Abdelstattar Ibrahem and Omnia Ahmed and Reham Abdaleem},
journal = {The Egyptian Journal of Histology},
year = {2026},
month = oct,
doi = {10.21608/ejh.2026.526639.2529},
url = {https://doi.org/10.21608/ejh.2026.526639.2529},
note = {CC BY},
}
RIS
TY - JOUR
TI - Nigella Sativa Oil versus Thyroxine in Carbimazole-Induced Hypothyroidism: Modulation of Oxidative Stress and Inflammatory Pathways
AU - Walaa Sayed
AU - Abdelstattar Ibrahem
AU - Omnia Ahmed
AU - Reham Abdaleem
PY - 2026
DA - 2026/10/25
JO - The Egyptian Journal of Histology
DO - 10.21608/ejh.2026.526639.2529
UR - https://doi.org/10.21608/ejh.2026.526639.2529
AB - Background: Reduction of thyroid hormone levels is known as hypothyroidism produces an alterable effect of bodily activities more willingly than a hormonal deficit. Aim of the Work: The present research was designed to compare the valuable influences of Nigella Sativa oil (NSO) and thyroxine on carbimazole-persuaded hypothyroidism. Materials and Methods: Forty rats were utilized and allocated into five groups. Group I (without treatment), included subgroups Ia and Ib were. Group II received distilled water, orally, included subgroups IIa and IIb. Group III was administered oral carbimazole, daily for three weeks (subgroup IIIa) and subgroup IIIb (recovered for an additional four weeks). All subgroups a and b were sacrificed after three and seven weeks, respectively. Group IV received carbimazole, like group III, followed by four weeks of daily oral NSO. Group V was given carbimazole like group III, followed by four weeks of oral thyroxine, daily. Serum levels of T3, T4, and TSH as well as oxidative markers in tissue; malondialdehyde and reduced glutathione were analyzed biochemically. In addition to histological examination, Caspase 3 and IL 6 immunohistochemical evaluations were performed. Results: Either NSO or thyroxine produced modifications effects in carbimazole-provoked histopathological changes of the thyroid gland, as demonstrated by a significant decrease in Caspase 3 immuno-expression, with more substantial regulating influence linked to thyroxine. In addition, both medications showed anti-inflammatory consequences (a decrease in immunohistochemical IL 6) and antioxidant capability (reduction in malondialdehyde and rise in reduced glutathione). Both NSO and thyroxine improved the thyroid function, nonetheless an additional impact related to thyroxine. Conclusion: Either NSO or thyroxine have an outstanding capability to minimize carbimazole- provoked negative consequences on the thyroid gland by regulation of oxidation-reduction state, as well as effective inhibition of inflammatory responses; thyroxine was more effective.
SN - 2090-2417
SN - 1110-0559
ER -