Novel Biochemical Findings from Bothrops marajoensis Venom.
- 1Institute of Agricultural, Exact and Biological Sciences of Iturama, Federal University of Triângulo Mineiro, Iturama 38280-000, Minas Gerais, Brazil.
- 2Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 19040-903, São Paulo, Brazil.
- 3Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University, Araraquara 19060-900, São Paulo, Brazil.
Abstract
Bothrops marajoensis is a Brazilian pit viper whose venom remains poorly characterized compared with those of other medically important Bothrops species. In the present study, we performed a comparative biochemical characterization of B. marajoensis venom with six additional Bothrops venoms (B. alternatus, B. atrox, B. jararaca, B. jararacussu, B. moojeni, and B. neuwiedi). Venoms were analyzed by reversed-phase chromatography, electrophoresis, immunorecognition by commercial bothropic antivenom, phosphodiesterase activity, and their effects on the viability of differentiated C2C12 myotubes. Chromatographic and electrophoretic analyses revealed a largely conserved overall venom composition among species, with qualitative differences, although quantitative differences in protein relative abundance were evident. Bothropic antivenom recognized all venoms, indicating broad antigenic cross-reactivity despite venom variation. B. marajoensis exhibited intermediate phosphodiesterase (PDE) activity relative to other species evaluated, confirming functional variability among Bothrops venoms. In the cell viability assay, B. marajoensis displayed comparatively low apparent cytotoxicity toward differentiated C2C12 myotubes, maintaining higher cell viability than highly myotoxic venoms such as those of B. jararacussu and B. moojeni. These findings expand the biochemical characterization of B. marajoensis venom and demonstrate that, although venoms share the conserved toxin repertoire typical for Bothrops genus, quantitative differences influence its biological activities, preserving broad recognition by bothropic antivenom.
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BibTeX
@article{oliveira2026novel,
title = {{Novel Biochemical Findings from Bothrops marajoensis Venom.}},
author = {Isadora Oliveira and Isabela Ferreira and Beatriz Jacob and Emilly Ramos and Lorena Agati and Isadora Lino and Francielle Cordeiro and Felipe Costa and Eloise Filardi and Manuela Pucca and Eliane Arantes},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198524},
url = {https://europepmc.org/article/MED/42883771},
note = {CC BY},
}
RIS
TY - JOUR
TI - Novel Biochemical Findings from Bothrops marajoensis Venom.
AU - Isadora Oliveira
AU - Isabela Ferreira
AU - Beatriz Jacob
AU - Emilly Ramos
AU - Lorena Agati
AU - Isadora Lino
AU - Francielle Cordeiro
AU - Felipe Costa
AU - Eloise Filardi
AU - Manuela Pucca
AU - Eliane Arantes
PY - 2026
DA - 2026/09/24
JO - International journal of molecular sciences
DO - 10.3390/ijms27198524
UR - https://europepmc.org/article/MED/42883771
AB - Bothrops marajoensis is a Brazilian pit viper whose venom remains poorly characterized compared with those of other medically important Bothrops species. In the present study, we performed a comparative biochemical characterization of B. marajoensis venom with six additional Bothrops venoms (B. alternatus, B. atrox, B. jararaca, B. jararacussu, B. moojeni, and B. neuwiedi). Venoms were analyzed by reversed-phase chromatography, electrophoresis, immunorecognition by commercial bothropic antivenom, phosphodiesterase activity, and their effects on the viability of differentiated C2C12 myotubes. Chromatographic and electrophoretic analyses revealed a largely conserved overall venom composition among species, with qualitative differences, although quantitative differences in protein relative abundance were evident. Bothropic antivenom recognized all venoms, indicating broad antigenic cross-reactivity despite venom variation. B. marajoensis exhibited intermediate phosphodiesterase (PDE) activity relative to other species evaluated, confirming functional variability among Bothrops venoms. In the cell viability assay, B. marajoensis displayed comparatively low apparent cytotoxicity toward differentiated C2C12 myotubes, maintaining higher cell viability than highly myotoxic venoms such as those of B. jararacussu and B. moojeni. These findings expand the biochemical characterization of B. marajoensis venom and demonstrate that, although venoms share the conserved toxin repertoire typical for Bothrops genus, quantitative differences influence its biological activities, preserving broad recognition by bothropic antivenom.
SN - 1422-0067
ER -