Adenovirus as a Causative Agent of an Acute Gastroenteritis Outbreak in Salvador, Bahia, Brazil.

Abstract

IntroductionAcute gastroenteritis (AGE) is a cause of childhood morbidity and may be associated with human adenovirus (HAdV) infection. This study aimed to investigate the occurrence of HAdV in fecal samples from children with AGE in Salvador, Bahia, Brazil, as well as to molecularly characterize the identified strains and evaluate their cytopathic effects in cell culture.

MethodsA total of 131 samples were analyzed using the nested polymerase chain reaction (Nested-PCR) technique. Molecular characterization was performed through Sanger sequencing and phylogenetic analysis, while Vero cell cultures were used to investigate the occurrence of cytopathic effects.

ResultsOf the samples analyzed, 13 (9.9%) tested positive for HAdV, while 118 (90.1%) were negative. The highest detection frequency was observed in children aged three years or younger, particularly those under one year of age. Phylogenetic analysis identified variants related to HAdV-F41 and HAdV-B3, revealing genetic differences and distinct clustering patterns compared with reference sequences. In cell culture, cytopathic effects were observed four days after inoculation, although further analyses are required to confirm productive viral replication.

ConclusionThese findings demonstrate the presence of HAdV in the samples studied and contribute to understanding its genetic diversity and circulation in Salvador, highlighting the importance of epidemiological surveillance and further studies on its role in the etiology of childhood AGE.

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BibTeX RIS

BibTeX

@misc{vidal2026adenovirus,
  title = {{Adenovirus as a Causative Agent of an Acute Gastroenteritis Outbreak in Salvador, Bahia, Brazil.}},
  author = {B. Vidal and S. Neves and I. P. Santos and S. I. Sardi and G. S. Campos},
  howpublished = {medRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.19.26365929},
  url = {https://www.medrxiv.org/content/10.64898/2026.10.19.26365929v1},
  note = {CC BY},
}

RIS

TY  - UNPB
TI  - Adenovirus as a Causative Agent of an Acute Gastroenteritis Outbreak in Salvador, Bahia, Brazil.
AU  - B. Vidal
AU  - S. Neves
AU  - I. P. Santos
AU  - S. I. Sardi
AU  - G. S. Campos
PY  - 2026
DA  - 2026/10/19
PB  - medRxiv
DO  - 10.64898/2026.10.19.26365929
UR  - https://www.medrxiv.org/content/10.64898/2026.10.19.26365929v1
AB  - IntroductionAcute gastroenteritis (AGE) is a cause of childhood morbidity and may be associated with human adenovirus (HAdV) infection. This study aimed to investigate the occurrence of HAdV in fecal samples from children with AGE in Salvador, Bahia, Brazil, as well as to molecularly characterize the identified strains and evaluate their cytopathic effects in cell culture.  MethodsA total of 131 samples were analyzed using the nested polymerase chain reaction (Nested-PCR) technique. Molecular characterization was performed through Sanger sequencing and phylogenetic analysis, while Vero cell cultures were used to investigate the occurrence of cytopathic effects.  ResultsOf the samples analyzed, 13 (9.9%) tested positive for HAdV, while 118 (90.1%) were negative. The highest detection frequency was observed in children aged three years or younger, particularly those under one year of age. Phylogenetic analysis identified variants related to HAdV-F41 and HAdV-B3, revealing genetic differences and distinct clustering patterns compared with reference sequences. In cell culture, cytopathic effects were observed four days after inoculation, although further analyses are required to confirm productive viral replication.  ConclusionThese findings demonstrate the presence of HAdV in the samples studied and contribute to understanding its genetic diversity and circulation in Salvador, highlighting the importance of epidemiological surveillance and further studies on its role in the etiology of childhood AGE.
ER  -

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