ACTL7A Expression and Functional Characteristics of Sperm in Infertile Men: A Comparison Between High and Low DNA Damage Groups, A Cross Sectional Study
- 1ACECR Institute of Higher Education (Isfahan Branch), Isfahan, Iran
- 2Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
- 3Pooyesh & Rooyesh Fertility Center, Isfahan health center, Iran
Abstract
Background:: Male infertility accounts for nearly half of all infertility cases, worldwide, and is frequently associated with molecular abnormalities that cannot be detected by routine semen analysis. Actin-like protein 7A (ACTL7A), a testis-specific actin-related protein essential for spermatogenesis, acrosome integrity, and oocyte activation, has recently emerged as a potential regulator of sperm function. This study aimed to investigate the association between ACTL7A expression and sperm DNA integrity in infertile men.
Materials and Methods:: In this comparative cross-sectional study, sixty men were divided into two groups based on sperm DNA fragmentation index (DFI), measured by the sperm chromatin structure assay (SCSA): low DFI (<20%) and high DFI (>20%). Semen parameters, chromatin integrity (aniline blue, chromomycin A3, toluidine blue), and oxidative stress markers [dihydroethidium (DHE) fluorescence and total antioxidant capacity (TAC)], were evaluated. The expression level of ACTL7A was quantified by quantitative polymerase chain reaction (qPCR) and Western blot, and its subcellular localization was determined by immunofluorescence microscopy.
Results:: Men with high DFI exhibited significantly reduced sperm motility, elevated levels of reactive oxygen species (ROS), and markedly compromised sperm chromatin integrity, accompanied by decreased ACTL7A expression (P=0.013). No significant differences in sperm morphology or TAC were observed between the groups. Immunofluorescence localized ACTL7A to the equatorial and acrosomal regions of the sperm head. Notably, ACTL7A protein levels correlated strongly with progressive motility (r=0.82, P=0.012). Consistently, individuals with high sperm DNA damage displayed a pronounced reduction in ACTL7A expression, with RNA levels decreased by ~3.4-fold and protein levels by ~2.2-fold, compared to those with low DNA damage.
Conclusion:: ACTL7A may serve as a potential indicator of sperm DNA integrity and motility, warranting further validation as a biomarker.
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BibTeX
@article{karimi2026actl7a,
title = {{ACTL7A Expression and Functional Characteristics of Sperm in Infertile Men: A Comparison Between High and Low DNA Damage Groups, A Cross Sectional Study}},
author = {Yeganeh Karimi and Bahareh Nikoozar and Marziyeh Tavalaee and Rana Zamani and Sajedeh Sadat Nourian Najafabadi and Parnia Zehtab and Mohammad Hossein Nasr-Esfahani},
journal = {International Journal of Fertility \& Sterility},
year = {2026},
month = oct,
doi = {10.22074/ijfs.2025.2077461.1983},
url = {https://doi.org/10.22074/ijfs.2025.2077461.1983},
note = {CC BY-NC 3.0},
}
RIS
TY - JOUR
TI - ACTL7A Expression and Functional Characteristics of Sperm in Infertile Men: A Comparison Between High and Low DNA Damage Groups, A Cross Sectional Study
AU - Yeganeh Karimi
AU - Bahareh Nikoozar
AU - Marziyeh Tavalaee
AU - Rana Zamani
AU - Sajedeh Sadat Nourian Najafabadi
AU - Parnia Zehtab
AU - Mohammad Hossein Nasr-Esfahani
PY - 2026
DA - 2026/10/03
JO - International Journal of Fertility & Sterility
DO - 10.22074/ijfs.2025.2077461.1983
UR - https://doi.org/10.22074/ijfs.2025.2077461.1983
AB - Background:: Male infertility accounts for nearly half of all infertility cases, worldwide, and is frequently associated with molecular abnormalities that cannot be detected by routine semen analysis. Actin-like protein 7A (ACTL7A), a testis-specific actin-related protein essential for spermatogenesis, acrosome integrity, and oocyte activation, has recently emerged as a potential regulator of sperm function. This study aimed to investigate the association between ACTL7A expression and sperm DNA integrity in infertile men. Materials and Methods:: In this comparative cross-sectional study, sixty men were divided into two groups based on sperm DNA fragmentation index (DFI), measured by the sperm chromatin structure assay (SCSA): low DFI (<20%) and high DFI (>20%). Semen parameters, chromatin integrity (aniline blue, chromomycin A3, toluidine blue), and oxidative stress markers [dihydroethidium (DHE) fluorescence and total antioxidant capacity (TAC)], were evaluated. The expression level of ACTL7A was quantified by quantitative polymerase chain reaction (qPCR) and Western blot, and its subcellular localization was determined by immunofluorescence microscopy. Results:: Men with high DFI exhibited significantly reduced sperm motility, elevated levels of reactive oxygen species (ROS), and markedly compromised sperm chromatin integrity, accompanied by decreased ACTL7A expression (P=0.013). No significant differences in sperm morphology or TAC were observed between the groups. Immunofluorescence localized ACTL7A to the equatorial and acrosomal regions of the sperm head. Notably, ACTL7A protein levels correlated strongly with progressive motility (r=0.82, P=0.012). Consistently, individuals with high sperm DNA damage displayed a pronounced reduction in ACTL7A expression, with RNA levels decreased by ~3.4-fold and protein levels by ~2.2-fold, compared to those with low DNA damage. Conclusion:: ACTL7A may serve as a potential indicator of sperm DNA integrity and motility, warranting further validation as a biomarker.
SN - 2008-076X
SN - 2008-0778
ER -