Peer-reviewedCC BYSTAR protocols
Protocol for integrated phosphoproteomic and proteomic analysis of the mouse ventral tegmental area with functional and kinase motif enrichment.
- 1Comprehensive Center for Pain and Addiction (CCPA), University of Arizona, Tucson, AZ 85724, USA; Department of Pharmacology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA.
- 2Department of Medicine, Division of Endocrinology, University of Arizona, Tucson, AZ 85724, USA. Electronic address: [email protected].
Abstract
We present a protocol for label-free proteomic and phosphoproteomic analysis of the mouse ventral tegmental area (VTA). We describe steps for combining region-specific biopsy-punch collection, parallel proteomic and phosphoproteomic processing, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and downstream enrichment analysis. We detail procedures for SynGO-based synaptic localization, g:Profiler/DAVID pathway enrichment, and iceLogo/pLogo motif analysis. This protocol enables quantitative assessment of protein abundance, phosphorylation dynamics, and functional interpretation from limited brain region samples. For complete details on the use and execution of this protocol, please refer to Diezel et al.¹.
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BibTeX
@article{diezel2026protocol,
title = {{Protocol for integrated phosphoproteomic and proteomic analysis of the mouse ventral tegmental area with functional and kinase motif enrichment.}},
author = {Cody C Diezel and Paul R Langlais and Todd W Vanderah and Arthur C Riegel},
journal = {STAR protocols},
year = {2026},
month = oct,
doi = {10.1016/j.xpro.2026.104911},
url = {https://europepmc.org/article/MED/42891946},
note = {CC BY},
}
RIS
TY - JOUR
TI - Protocol for integrated phosphoproteomic and proteomic analysis of the mouse ventral tegmental area with functional and kinase motif enrichment.
AU - Cody C Diezel
AU - Paul R Langlais
AU - Todd W Vanderah
AU - Arthur C Riegel
PY - 2026
DA - 2026/10/17
JO - STAR protocols
DO - 10.1016/j.xpro.2026.104911
UR - https://europepmc.org/article/MED/42891946
AB - We present a protocol for label-free proteomic and phosphoproteomic analysis of the mouse ventral tegmental area (VTA). We describe steps for combining region-specific biopsy-punch collection, parallel proteomic and phosphoproteomic processing, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and downstream enrichment analysis. We detail procedures for SynGO-based synaptic localization, g:Profiler/DAVID pathway enrichment, and iceLogo/pLogo motif analysis. This protocol enables quantitative assessment of protein abundance, phosphorylation dynamics, and functional interpretation from limited brain region samples. For complete details on the use and execution of this protocol, please refer to Diezel et al.¹.
SN - 2666-1667
ER -