Phenolic Profiling of Grapevine Leaves and the Protective Effect of a Polyphenol-Rich Vitis amurensis Extract on Lactiplantibacillus plantarum Under Oxidative Stress.
5 affiliations
- 1Research Institute for Viticulture and Oenology, University of Pécs, 7634 Pécs, Hungary.
- 2Cochrane Hungary, Medical School, University of Pécs, 7624 Pécs, Hungary.
- 3Julius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, 76833 Siebeldingen, Germany.
- 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, 7624 Pécs, Hungary.
- 5Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, 7624 Pécs, Hungary.
Abstract
Polyphenols are plant secondary metabolites widely recognized for their antioxidant properties and potential health benefits. Grapevine leaves are a rich yet underexplored source of these bioactive compounds. In this study, caftaric acid, and predominant flavonol glycosides were characterized in leaves of seventeen grapevine genotypes by high-performance liquid chromatography, while total phenolic content (TPC) was determined using the Folin-Ciocalteu assay. In addition, the growth-promoting effect of a polyphenol-rich Vitis amurensis leaf extract on Lactiplantibacillus plantarum and its protective effect under H₂O₂-induced oxidative stress were evaluated. Both TPC and phenolic composition were strongly genotype-dependent, with TPC ranging from approximately 20 to 100 mg gallic acid equivalents (GAE) g⁻¹ dry weight (DW). Quercetin and kaempferol glycosides were the predominant flavonols, and quercetin-3-O-glucuronide, one of the most abundant derivatives, varied nearly tenfold among genotypes (approximately 2100-20,000 mg kg⁻¹ DW). The leaf extract of V. amurensis, the genotype with the highest TPC, significantly promoted the in vitro growth of L. plantarum in a dose-dependent manner, resulting in higher viable cell counts after 24 h. The extract also markedly enhanced bacterial survival under H₂O₂-induced oxidative stress. At 2 mM H₂O₂, no bacterial growth was detected in the absence of the extract, whereas extract-supplemented cultures maintained substantial growth and reached a viable cell count of 1.8 × 10⁸ colony-forming units (CFU) mL⁻¹ after 24 h. The phenolic patterns identified across the investigated genotypes provide a basis for selecting Vitis genotypes as sources of specific flavonol glycosides. Furthermore, our microbiological results provide preliminary evidence of the beneficial effects of V. amurensis leaf extract on L. plantarum and warrant further investigation of its potential functional applications.
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BibTeX
@article{korosi2026phenolic,
title = {{Phenolic Profiling of Grapevine Leaves and the Protective Effect of a Polyphenol-Rich Vitis amurensis Extract on Lactiplantibacillus plantarum Under Oxidative Stress.}},
author = {László Kőrösi and Okba Hatem and Franco Röckel and György Schneider and Szilvia Papp},
journal = {Antioxidants (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/antiox15101266},
url = {https://europepmc.org/article/MED/42939840},
note = {CC BY},
}
RIS
TY - JOUR
TI - Phenolic Profiling of Grapevine Leaves and the Protective Effect of a Polyphenol-Rich Vitis amurensis Extract on Lactiplantibacillus plantarum Under Oxidative Stress.
AU - László Kőrösi
AU - Okba Hatem
AU - Franco Röckel
AU - György Schneider
AU - Szilvia Papp
PY - 2026
DA - 2026/10/01
JO - Antioxidants (Basel, Switzerland)
DO - 10.3390/antiox15101266
UR - https://europepmc.org/article/MED/42939840
AB - Polyphenols are plant secondary metabolites widely recognized for their antioxidant properties and potential health benefits. Grapevine leaves are a rich yet underexplored source of these bioactive compounds. In this study, caftaric acid, and predominant flavonol glycosides were characterized in leaves of seventeen grapevine genotypes by high-performance liquid chromatography, while total phenolic content (TPC) was determined using the Folin-Ciocalteu assay. In addition, the growth-promoting effect of a polyphenol-rich Vitis amurensis leaf extract on Lactiplantibacillus plantarum and its protective effect under H₂O₂-induced oxidative stress were evaluated. Both TPC and phenolic composition were strongly genotype-dependent, with TPC ranging from approximately 20 to 100 mg gallic acid equivalents (GAE) g⁻¹ dry weight (DW). Quercetin and kaempferol glycosides were the predominant flavonols, and quercetin-3-O-glucuronide, one of the most abundant derivatives, varied nearly tenfold among genotypes (approximately 2100-20,000 mg kg⁻¹ DW). The leaf extract of V. amurensis, the genotype with the highest TPC, significantly promoted the in vitro growth of L. plantarum in a dose-dependent manner, resulting in higher viable cell counts after 24 h. The extract also markedly enhanced bacterial survival under H₂O₂-induced oxidative stress. At 2 mM H₂O₂, no bacterial growth was detected in the absence of the extract, whereas extract-supplemented cultures maintained substantial growth and reached a viable cell count of 1.8 × 10⁸ colony-forming units (CFU) mL⁻¹ after 24 h. The phenolic patterns identified across the investigated genotypes provide a basis for selecting Vitis genotypes as sources of specific flavonol glycosides. Furthermore, our microbiological results provide preliminary evidence of the beneficial effects of V. amurensis leaf extract on L. plantarum and warrant further investigation of its potential functional applications.
SN - 2076-3921
ER -