Fluorescence Sensing of Anions Like Adenosine 5ʹ‐Triphosphate With pH‐Gated Porous Dye‐Loaded Cerasomes
- 1Chemical and Optical Sensing Division Bundesanstalt für Materialforschung und ‐prüfung (BAM) Berlin Germany
Abstract
The development of gated systems capable of controlled opening of pores on demand represents an attractive way to control chemical functions in many fields of application. Here, we investigate the loading of various reporter dyes into non‐porous and porous cerasomes, a hybrid nanoscopic biomimetic material, and how pH‐dependent supramolecular adenosine 5′‐triphosphate (ATP) binding at the polyaminic gatekeepers anchored at the cerasomes’ pore outlets is influencing pH gating. Such organic‐inorganic nanovesicles are composed of a siliceous crosslinked lipid bilayer enclosing a liquid‐filled void. Preparation of porous cerasomes was achieved by inserting poloxamer block‐co‐polymers into the membrane during synthesis. To our knowledge, this is the first example of porous cerasomes and their use in gating applications. We successfully encapsulated or loaded reporter molecules into these objects, with the loading preference depending on their charge, hydrophobicity and chemical structure. The addition of molecular gates, responding to pH and/or anions such as ATP, allowed the pores’ controlled opening, thereby modulating proton influx, which can be read out via fluorescence response. Considering the corresponding pH sensitivity, dye loaded cerasomes are promising reagents for detecting ATP in relevant concentrations (0.02 to 1000 µm) with distinctly faster fluorescence responses as compared to standard gated mesoporous materials.
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BibTeX
@article{vinot2026fluorescence,
title = {{Fluorescence Sensing of Anions Like Adenosine 5ʹ‐Triphosphate With pH‐Gated Porous Dye‐Loaded Cerasomes}},
author = {Romane Vinot and Estela Climent and Maja Friedrich and Jérémy Bell and Knut Rurack},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
year = {2026},
month = aug,
doi = {10.1002/smll.75507},
url = {https://doi.org/10.1002/smll.75507},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Fluorescence Sensing of Anions Like Adenosine 5ʹ‐Triphosphate With pH‐Gated Porous Dye‐Loaded Cerasomes
AU - Romane Vinot
AU - Estela Climent
AU - Maja Friedrich
AU - Jérémy Bell
AU - Knut Rurack
PY - 2026
DA - 2026/08/31
JO - Small (Weinheim an Der Bergstrasse, Germany)
DO - 10.1002/smll.75507
UR - https://doi.org/10.1002/smll.75507
AB - The development of gated systems capable of controlled opening of pores on demand represents an attractive way to control chemical functions in many fields of application. Here, we investigate the loading of various reporter dyes into non‐porous and porous cerasomes, a hybrid nanoscopic biomimetic material, and how pH‐dependent supramolecular adenosine 5′‐triphosphate (ATP) binding at the polyaminic gatekeepers anchored at the cerasomes’ pore outlets is influencing pH gating. Such organic‐inorganic nanovesicles are composed of a siliceous crosslinked lipid bilayer enclosing a liquid‐filled void. Preparation of porous cerasomes was achieved by inserting poloxamer block‐co‐polymers into the membrane during synthesis. To our knowledge, this is the first example of porous cerasomes and their use in gating applications. We successfully encapsulated or loaded reporter molecules into these objects, with the loading preference depending on their charge, hydrophobicity and chemical structure. The addition of molecular gates, responding to pH and/or anions such as ATP, allowed the pores’ controlled opening, thereby modulating proton influx, which can be read out via fluorescence response. Considering the corresponding pH sensitivity, dye loaded cerasomes are promising reagents for detecting ATP in relevant concentrations (0.02 to 1000 µm) with distinctly faster fluorescence responses as compared to standard gated mesoporous materials.
SN - 1613-6810
SN - 1613-6829
ER -