Influence of dentin depth and hemostatic contamination on microtensile bond strength of a universal adhesive: Effect of phosphoric acid re-etching as a rescue protocol.
- 1Department of Conservative Dentistry and Endodontics, Kalinga Institute of Dental Sciences (KIDS), KIIT Deemed to be University, Bhubaneswar, 751024, Odisha, India.
Abstract
Background
This study evaluated the influence of dentin depth and surface treatment on the microtensile bond strength (µTBS) of a universal adhesive to dentin contaminated with an aluminum chloride hemostatic agent, and assessed the effectiveness of phosphoric acid re-etching as a contamination management strategy.Materials and methods
Sixty extracted human third molars were assigned to six groups (n=10) based on dentin depth (superficial or deep) and surface treatment (control, contamination, and contamination followed by phosphoric acid re-etching). A universal adhesive was applied in self-etch mode for control and contamination groups, and in etch-and-rinse mode following the rescue protocol. Composite build-up was performed and specimens were thermocycled (10,000 cycles). µTBS was measured using a universal testing machine. Data were analyzed using two-way ANOVA and Tukey's post hoc test (p<0.05). Failure modes were evaluated using stereomicroscopy and analyzed with Pearson's chi-square test.Results
Superficial dentin demonstrated significantly higher µTBS than deep dentin (p<0.001). Aluminum chloride contamination significantly reduced bond strength in both substrates (p<0.05). Phosphoric acid re-etching significantly improved bond strength in superficial dentin (p<0.05) but did not restore control values. In deep dentin, the rescue protocol did not significantly improve bond strength (p>0.05). A significant interaction between dentin depth and surface treatment was observed (p=0.001).Conclusions
Dentin depth significantly influences the effectiveness of contamination management strategies. Phosphoric acid re-etching partially restores bond strength in superficial dentin but is ineffective in deep dentin, indicating that contamination management is substrate-dependent.
Related
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BibTeX
@article{patri2026influence,
title = {{Influence of dentin depth and hemostatic contamination on microtensile bond strength of a universal adhesive: Effect of phosphoric acid re-etching as a rescue protocol.}},
author = {Gaurav Patri and Debkant Jena and Prasanti Kumari Pradhan and Pratik Agrawal and Akansha Tilokani and Swadheena Patro},
journal = {Journal of clinical and experimental dentistry},
year = {2026},
month = aug,
doi = {10.4317/jced.64286},
url = {https://europepmc.org/article/MED/42894718},
note = {CC BY},
}
RIS
TY - JOUR
TI - Influence of dentin depth and hemostatic contamination on microtensile bond strength of a universal adhesive: Effect of phosphoric acid re-etching as a rescue protocol.
AU - Gaurav Patri
AU - Debkant Jena
AU - Prasanti Kumari Pradhan
AU - Pratik Agrawal
AU - Akansha Tilokani
AU - Swadheena Patro
PY - 2026
DA - 2026/08/28
JO - Journal of clinical and experimental dentistry
DO - 10.4317/jced.64286
UR - https://europepmc.org/article/MED/42894718
AB - Background This study evaluated the influence of dentin depth and surface treatment on the microtensile bond strength (µTBS) of a universal adhesive to dentin contaminated with an aluminum chloride hemostatic agent, and assessed the effectiveness of phosphoric acid re-etching as a contamination management strategy.Materials and methods Sixty extracted human third molars were assigned to six groups (n=10) based on dentin depth (superficial or deep) and surface treatment (control, contamination, and contamination followed by phosphoric acid re-etching). A universal adhesive was applied in self-etch mode for control and contamination groups, and in etch-and-rinse mode following the rescue protocol. Composite build-up was performed and specimens were thermocycled (10,000 cycles). µTBS was measured using a universal testing machine. Data were analyzed using two-way ANOVA and Tukey's post hoc test (p<0.05). Failure modes were evaluated using stereomicroscopy and analyzed with Pearson's chi-square test.Results Superficial dentin demonstrated significantly higher µTBS than deep dentin (p<0.001). Aluminum chloride contamination significantly reduced bond strength in both substrates (p<0.05). Phosphoric acid re-etching significantly improved bond strength in superficial dentin (p<0.05) but did not restore control values. In deep dentin, the rescue protocol did not significantly improve bond strength (p>0.05). A significant interaction between dentin depth and surface treatment was observed (p=0.001).Conclusions Dentin depth significantly influences the effectiveness of contamination management strategies. Phosphoric acid re-etching partially restores bond strength in superficial dentin but is ineffective in deep dentin, indicating that contamination management is substrate-dependent.
SN - 1989-5488
ER -