Development of loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick DNA biosensor for detection of α thalassemia 1 [Southeast Asia (SEA), --SEA deletion]
4 affiliations
- 1Department of Clinical Pathology, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok, Thailand
- 2Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
- 3Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
- 4Innovative Learning Center, Srinakharinwirot University, Thailand
Abstract
This study aimed to develop a one-step loop-mediated isothermal amplification combined with a lateral flow dipstick (LAMP-LFD) DNA biosensor for the detection of α0-thalassemia (SEA deletion). Genomic DNA was obtained from 48 α0-thalassemia (SEA deletion)-positive and 48 (SEA deletion)-negative (normal) Thai subjects. DNA was analyzed by LAMP assay at 61°C for 60 min using specific primers for the α0-thalassemia (SEA deletion) type. Following amplification, the products were visually detected using an LFD, with results available within 5–10 min. The one-step LAMP-LFD DNA biosensor could detect DNA carrying the α0-thalassemia (SEA deletion) type at levels as low as 200 pg, with 100% sensitivity and 100% specificity. Furthermore, no cross-reactivity was observed with DNA carrying other α0-thalassemia deletion types (THAI deletion). The LAMP-LFD DNA biosensor is useful for identifying carriers of α0-thalassemia (SEA deletion) (the most common deletion type found in Thailand and across Southeast Asia). This novel one-step LAMP-LFD kit will be beneficial for medical laboratory technologists performing genetic carrier screening tests as it is simple to perform and requires minimal equipment and time. Furthermore, this method supports clinicians in providing genetic counseling and implementing preventive strategies against severe thalassemia, a significant public health concern in Thailand.
Related
- Development of a multiplex detection system for toxic Amanita species based on isothermal recombinase polymerase amplification and CRISPR/Cas12b technology
- Colorimetric Detection of the vanA Gene in Vancomycin-Resistant Enterococcus Using Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
- Detection of Plasmodium vivax and Plasmodium falciparum using colorimetric loop-mediated isothermal amplification from dried blood spots collected in active and passive malaria surveillance in the Peruvian Amazon
- Isothermal Strand Displacement Amplification for Chemical Sensing: From Molecular Design to Integrated and Intelligent Platforms.
- Browser-based design of loop-mediated isothermal amplification (LAMP) primer sets with template repeat masking and bisulphite support
BibTeX
@article{ckumdee2026development,
title = {{Development of loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick DNA biosensor for detection of α thalassemia 1 [Southeast Asia (SEA), --SEA deletion]}},
author = {Jutturong Ckumdee and Sataron Karnjanaopas and Duantida Songdej and Noppawan Tungbuppha and Thongchai Kaewphinit and Thanwa Wongsuk and Chanita Sanyear},
journal = {PLOS One},
year = {2026},
month = oct,
doi = {10.1371/journal.pone.0360555},
url = {https://doi.org/10.1371/journal.pone.0360555},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Development of loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick DNA biosensor for detection of α thalassemia 1 [Southeast Asia (SEA), --SEA deletion]
AU - Jutturong Ckumdee
AU - Sataron Karnjanaopas
AU - Duantida Songdej
AU - Noppawan Tungbuppha
AU - Thongchai Kaewphinit
AU - Thanwa Wongsuk
AU - Chanita Sanyear
PY - 2026
DA - 2026/10/12
JO - PLOS One
DO - 10.1371/journal.pone.0360555
UR - https://doi.org/10.1371/journal.pone.0360555
AB - This study aimed to develop a one-step loop-mediated isothermal amplification combined with a lateral flow dipstick (LAMP-LFD) DNA biosensor for the detection of α0-thalassemia (SEA deletion). Genomic DNA was obtained from 48 α0-thalassemia (SEA deletion)-positive and 48 (SEA deletion)-negative (normal) Thai subjects. DNA was analyzed by LAMP assay at 61°C for 60 min using specific primers for the α0-thalassemia (SEA deletion) type. Following amplification, the products were visually detected using an LFD, with results available within 5–10 min. The one-step LAMP-LFD DNA biosensor could detect DNA carrying the α0-thalassemia (SEA deletion) type at levels as low as 200 pg, with 100% sensitivity and 100% specificity. Furthermore, no cross-reactivity was observed with DNA carrying other α0-thalassemia deletion types (THAI deletion). The LAMP-LFD DNA biosensor is useful for identifying carriers of α0-thalassemia (SEA deletion) (the most common deletion type found in Thailand and across Southeast Asia). This novel one-step LAMP-LFD kit will be beneficial for medical laboratory technologists performing genetic carrier screening tests as it is simple to perform and requires minimal equipment and time. Furthermore, this method supports clinicians in providing genetic counseling and implementing preventive strategies against severe thalassemia, a significant public health concern in Thailand.
SN - 1932-6203
ER -