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Peer-reviewedCC BY 4.0Microbiology Spectrum

Version 2.0 of the Hermes transposon-based mutagenesis system for prototrophic mutant pool generation in Candida glabrata

  • Junhua Wan1
  • Yuru Guo1
  • Yabing Song1
  • Jiaxin Gao1
  1. 1State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
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Abstract

Although the original Hermes transposon mutagenesis system has proven successful for saturated insertional mutagenesis in Candida glabrata, its reliance on uracil auxotrophy may limit its applicability under nutrient-restricted host environments. To overcome this limitation, Zaeske et al. (Microbiol Spectr 14:e0084826, 2026, https://doi.org/10.1128/spectrum.00848-26) recently developed a prototrophic alternative that uses cycloheximide (CHX) instead of 5-fluoroorotic acid (5-FOA) to enrich for transposon insertion mutants, generating high-complexity pools. This system provides a valuable resource for investigating the genetic and cellular determinants of colonization and pathogenicity in this important fungal pathogen.

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Cite

BibTeXRIS
BibTeX
@article{wan2026version,
  title = {{Version 2.0 of the Hermes transposon-based mutagenesis system for prototrophic mutant pool generation in Candida glabrata}},
  author = {Junhua Wan and Yuru Guo and Yabing Song and Jiaxin Gao},
  journal = {Microbiology Spectrum},
  year = {2026},
  month = sep,
  doi = {10.1128/spectrum.02529-26},
  url = {https://doi.org/10.1128/spectrum.02529-26},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Version 2.0 of the Hermes transposon-based mutagenesis system for prototrophic mutant pool generation in Candida glabrata
AU  - Junhua Wan
AU  - Yuru Guo
AU  - Yabing Song
AU  - Jiaxin Gao
PY  - 2026
DA  - 2026/09/28
JO  - Microbiology Spectrum
DO  - 10.1128/spectrum.02529-26
UR  - https://doi.org/10.1128/spectrum.02529-26
AB  - Although the original Hermes transposon mutagenesis system has proven successful for saturated insertional mutagenesis in Candida glabrata, its reliance on uracil auxotrophy may limit its applicability under nutrient-restricted host environments. To overcome this limitation, Zaeske et al. (Microbiol Spectr 14:e0084826, 2026, https://doi.org/10.1128/spectrum.00848-26) recently developed a prototrophic alternative that uses cycloheximide (CHX) instead of 5-fluoroorotic acid (5-FOA) to enrich for transposon insertion mutants, generating high-complexity pools. This system provides a valuable resource for investigating the genetic and cellular determinants of colonization and pathogenicity in this important fungal pathogen.
SN  - 2165-0497
ER  -