Serodiagnosis of Human Strongyloidiasis in Brazil and Peru Using Strongyloides venezuelensis Antigens: A Comparative Study in Endemic Settings.
- 1Laboratório de Investigação Médica - LIM/06 (Imunopatologia da Esquistossomose) do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
- 2Laboratorio de Parasitología, Instituto de Medicina Tropical "Alexander Von Humboldt", Universidad Peruana Cayetano Heredia, Lima, Perú.
Abstract
Strongyloidiasis is a neglected tropical disease caused by the soil-transmitted helminth Strongyloides stercoralis. Immunological assays employing heterologous antigens from Strongyloides venezuelensis represent a valuable alternative to parasitological diagnosis. In this study, we evaluated the diagnostic performance of different antigenic preparations derived from S. venezuelensis infective larvae (L3) for the detection of anti-Strongyloides IgG antibodies in sera from Brazilian and Peruvian individuals. Total soluble (S-L3), membrane (M-L3), cuticular (C-L3) and excretory/secretory (ES-L3) antigenic extracts were assessed using enzyme-linked immunosorbent assay (ELISA). Overall, Brazilian sera showed higher reactivity than Peruvian sera to all antigenic preparations. Both S-iL3 and ES-L3 demonstrated excellent diagnostic performance with samples from both countries, showing 100% sensitivity and specificities of 97.4% and 100%, respectively. When a single cut-off value applicable to both populations was adopted, S-L3 showed the best overall performance, achieving 100% sensitivity and 98.7% specificity at a cut-off of 0.367, or 97.5% sensitivity and 100% specificity at a cut-off of 0.416, followed by ES-L3, C-L3 and M-L3. Heterologous antigenic extracts from S. venezuelensis, particularly S-L3, may represent a useful alternative for the serological diagnosis of S. stercoralis infection in endemic settings such as Brazil and Peru.
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BibTeX
@article{roldan2026serodiagnosis,
title = {{Serodiagnosis of Human Strongyloidiasis in Brazil and Peru Using Strongyloides venezuelensis Antigens: A Comparative Study in Endemic Settings.}},
author = {William Henry Roldán and Marcelo Andreetta Corral and Dirce Mary Correia Lima Meisel and Marco Canales and Maria Angelica Terashima and Silvia Vanessa Lourenço and Ronaldo Cesar Borges Gryschek and Fabiana Martins de Paula},
journal = {Parasite immunology},
year = {2026},
month = oct,
doi = {10.1111/pim.70109},
url = {https://europepmc.org/article/MED/42906626},
note = {CC BY},
}
RIS
TY - JOUR
TI - Serodiagnosis of Human Strongyloidiasis in Brazil and Peru Using Strongyloides venezuelensis Antigens: A Comparative Study in Endemic Settings.
AU - William Henry Roldán
AU - Marcelo Andreetta Corral
AU - Dirce Mary Correia Lima Meisel
AU - Marco Canales
AU - Maria Angelica Terashima
AU - Silvia Vanessa Lourenço
AU - Ronaldo Cesar Borges Gryschek
AU - Fabiana Martins de Paula
PY - 2026
DA - 2026/10/01
JO - Parasite immunology
DO - 10.1111/pim.70109
UR - https://europepmc.org/article/MED/42906626
AB - Strongyloidiasis is a neglected tropical disease caused by the soil-transmitted helminth Strongyloides stercoralis. Immunological assays employing heterologous antigens from Strongyloides venezuelensis represent a valuable alternative to parasitological diagnosis. In this study, we evaluated the diagnostic performance of different antigenic preparations derived from S. venezuelensis infective larvae (L3) for the detection of anti-Strongyloides IgG antibodies in sera from Brazilian and Peruvian individuals. Total soluble (S-L3), membrane (M-L3), cuticular (C-L3) and excretory/secretory (ES-L3) antigenic extracts were assessed using enzyme-linked immunosorbent assay (ELISA). Overall, Brazilian sera showed higher reactivity than Peruvian sera to all antigenic preparations. Both S-iL3 and ES-L3 demonstrated excellent diagnostic performance with samples from both countries, showing 100% sensitivity and specificities of 97.4% and 100%, respectively. When a single cut-off value applicable to both populations was adopted, S-L3 showed the best overall performance, achieving 100% sensitivity and 98.7% specificity at a cut-off of 0.367, or 97.5% sensitivity and 100% specificity at a cut-off of 0.416, followed by ES-L3, C-L3 and M-L3. Heterologous antigenic extracts from S. venezuelensis, particularly S-L3, may represent a useful alternative for the serological diagnosis of S. stercoralis infection in endemic settings such as Brazil and Peru.
SN - 0141-9838
SN - 1365-3024
ER -