Time- and Concentration-dependent Profiles of Cell Viability and Pro-tumorigenic Gene Expression Following Interleukin-8 Stimulation in A549 Lung Cancer Cells.
- 1Department of Pharmacology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.
Abstract
Background
Lung cancer is closely linked to inflammation, with interleukins such as interleukin-8 (IL-8) playing a key role in disease development and progression. While IL-8 has been implicated in tumor-promoting processes, its direct effects on lung cancer cell behavior remain incompletely characterized.Objective
To assess the impact of IL-8 on cell viability and mRNA expression of pro-tumorigenic markers in A549 cells.Materials and methods
Cell viability was measured using MTT assay. The mRNA expression levels of matrix metalloproteinase-7 (MMP-7), C-X-C motif chemokine ligand 1 (CXCL1), and interleukin-1α (IL-1α) were examined by RT-qPCR.Results
Two-way analysis revealed significant main effects for both timepoint and concentration on cell viability. Post hoc testing showed that IL-8 stimulation (0.5 ng/mL) induced a significant increase in A549 cell viability at the 24-hour timepoint. In contrast, prolonged stimulation (0.5 ng/mL) at 48 hours resulted in a marked downward trend in cell viability, followed by a return to baseline levels at 72 hours, indicating a highly dynamic, time-dependent cellular profile. At the transcriptional level, an initial upward trend in MMP-7 gene expression at a low concentration was not statistically significant following variance stabilization. Similarly, no significant changes in CXCL1 or IL-1α expression profiles were observed across concentrations.Conclusion
IL-8 was found to exert selective, context-dependent effects on A549 lung cancer cells, driving early viability shifts and an upregulatory trend in MMP-7 expression. These findings underscore the nuanced role of IL-8 in the tumor microenvironment and warrant future functional assays to fully elucidate its underlying mechanisms in lung cancer.
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BibTeX
@article{ibrahim2026time,
title = {{Time- and Concentration-dependent Profiles of Cell Viability and Pro-tumorigenic Gene Expression Following Interleukin-8 Stimulation in A549 Lung Cancer Cells.}},
author = {Zaridatul Aini Ibrahim and Ain Khadija Arif and Suha Azizan and Zher Pin Goh and Muhammad Farid Nazer Muhammad Faruqu},
journal = {Saudi journal of medicine \& medical sciences},
year = {2026},
month = oct,
doi = {10.4103/sjmms.sjmms_343_26},
url = {https://europepmc.org/article/MED/42974303},
note = {CC BY-NC-SA},
}
RIS
TY - JOUR
TI - Time- and Concentration-dependent Profiles of Cell Viability and Pro-tumorigenic Gene Expression Following Interleukin-8 Stimulation in A549 Lung Cancer Cells.
AU - Zaridatul Aini Ibrahim
AU - Ain Khadija Arif
AU - Suha Azizan
AU - Zher Pin Goh
AU - Muhammad Farid Nazer Muhammad Faruqu
PY - 2026
DA - 2026/10/14
JO - Saudi journal of medicine & medical sciences
DO - 10.4103/sjmms.sjmms_343_26
UR - https://europepmc.org/article/MED/42974303
AB - Background Lung cancer is closely linked to inflammation, with interleukins such as interleukin-8 (IL-8) playing a key role in disease development and progression. While IL-8 has been implicated in tumor-promoting processes, its direct effects on lung cancer cell behavior remain incompletely characterized.Objective To assess the impact of IL-8 on cell viability and mRNA expression of pro-tumorigenic markers in A549 cells.Materials and methods Cell viability was measured using MTT assay. The mRNA expression levels of matrix metalloproteinase-7 (MMP-7), C-X-C motif chemokine ligand 1 (CXCL1), and interleukin-1α (IL-1α) were examined by RT-qPCR.Results Two-way analysis revealed significant main effects for both timepoint and concentration on cell viability. Post hoc testing showed that IL-8 stimulation (0.5 ng/mL) induced a significant increase in A549 cell viability at the 24-hour timepoint. In contrast, prolonged stimulation (0.5 ng/mL) at 48 hours resulted in a marked downward trend in cell viability, followed by a return to baseline levels at 72 hours, indicating a highly dynamic, time-dependent cellular profile. At the transcriptional level, an initial upward trend in MMP-7 gene expression at a low concentration was not statistically significant following variance stabilization. Similarly, no significant changes in CXCL1 or IL-1α expression profiles were observed across concentrations.Conclusion IL-8 was found to exert selective, context-dependent effects on A549 lung cancer cells, driving early viability shifts and an upregulatory trend in MMP-7 expression. These findings underscore the nuanced role of IL-8 in the tumor microenvironment and warrant future functional assays to fully elucidate its underlying mechanisms in lung cancer.
SN - 1658-631X
SN - 2321-4856
ER -