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Peer-reviewedCC BY 4.0International Journal of Molecular Sciences

Transcriptomic and Metabolomic Investigation of Cell Wall Remodeling and Hormonal Signaling Dysfunction in Short Silique Mutant Rapeseed

  • Menglin Zhou1
  • Xi Song1,2
  • Bingbing Dai1
  • Wei Zhou1
  • Xiaofei Zan1
  • Qijin Jing1
  • Qingqing Yu1
  • Wuming Deng1
  1. 1Nanchong Academy of Agricultural Sciences, Nanchong 637000, China
  2. 2College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
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Abstract

Silique development is a critical factor influencing the yield of rapeseed (B. napus). This study employed the CRISPR/Cas9-mediated BnMYB46 knockout mutant K46 to investigate the molecular mechanisms underlying its short-silique phenotype via integrated cytological, transcriptomic, untargeted metabolomic, and targeted hormone metabolomic analyses. The K46 mutant exhibited significantly reduced silique length, seed number per silique, and dry weight. Cytological observations revealed profound folding of epidermal cells and severe compression of parenchyma cells, consistent with impaired cell expansion. Multi-omics analysis identified plant hormone signal transduction as a core hub pathway disrupted at 20 DAF, and targeted metabolomics confirmed significant endogenous IAA and GA imbalances. qRT-PCR validation showed key hormone genes were significantly altered in K46, with decreases of 22.3-fold in IAA2 and 18.8-fold in GA3OX1 at 20 DAF, and a 165-fold increase in ABF4. These early hormonal disruptions drove downstream suppression of cell wall synthesis and carbohydrate metabolism pathways, leading to impaired cell expansion and compromised structural integrity. This study provides new insights into the hormonal regulatory network governing silique development and identifies potential targets for the molecular improvement of silique-related traits in rapeseed.

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Cite

BibTeXRIS
BibTeX
@article{zhou2026transcriptomic,
  title = {{Transcriptomic and Metabolomic Investigation of Cell Wall Remodeling and Hormonal Signaling Dysfunction in Short Silique Mutant Rapeseed}},
  author = {Menglin Zhou and Xi Song and Bingbing Dai and Wei Zhou and Xiaofei Zan and Qijin Jing and Qingqing Yu and Wuming Deng},
  journal = {International Journal of Molecular Sciences},
  year = {2026},
  month = sep,
  doi = {10.3390/ijms27188349},
  url = {https://www.mdpi.com/1422-0067/27/18/8349},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Transcriptomic and Metabolomic Investigation of Cell Wall Remodeling and Hormonal Signaling Dysfunction in Short Silique Mutant Rapeseed
AU  - Menglin Zhou
AU  - Xi Song
AU  - Bingbing Dai
AU  - Wei Zhou
AU  - Xiaofei Zan
AU  - Qijin Jing
AU  - Qingqing Yu
AU  - Wuming Deng
PY  - 2026
DA  - 2026/09/19
JO  - International Journal of Molecular Sciences
DO  - 10.3390/ijms27188349
UR  - https://www.mdpi.com/1422-0067/27/18/8349
AB  - Silique development is a critical factor influencing the yield of rapeseed (B. napus). This study employed the CRISPR/Cas9-mediated BnMYB46 knockout mutant K46 to investigate the molecular mechanisms underlying its short-silique phenotype via integrated cytological, transcriptomic, untargeted metabolomic, and targeted hormone metabolomic analyses. The K46 mutant exhibited significantly reduced silique length, seed number per silique, and dry weight. Cytological observations revealed profound folding of epidermal cells and severe compression of parenchyma cells, consistent with impaired cell expansion. Multi-omics analysis identified plant hormone signal transduction as a core hub pathway disrupted at 20 DAF, and targeted metabolomics confirmed significant endogenous IAA and GA imbalances. qRT-PCR validation showed key hormone genes were significantly altered in K46, with decreases of 22.3-fold in IAA2 and 18.8-fold in GA3OX1 at 20 DAF, and a 165-fold increase in ABF4. These early hormonal disruptions drove downstream suppression of cell wall synthesis and carbohydrate metabolism pathways, leading to impaired cell expansion and compromised structural integrity. This study provides new insights into the hormonal regulatory network governing silique development and identifies potential targets for the molecular improvement of silique-related traits in rapeseed.
SN  - 1422-0067
ER  -