Dataset of amplicons, metagenomes, and metagenome-assembled genomes from geothermal biofilms four years after a volcanic eruption in Hawai'i.
4 affiliations
- 1Te Aka Mātuatua - School of Science, Te Whare Wānanga o Waikato - University of Waikato, Hamilton, New Zealand.
- 2International Centre for Terrestrial Antarctic Research, University of Waikato, Hamilton, New Zealand.
- 3Tecnológico Nacional de Mexico Campus Veracruz, Veracruz, Mexico.
- 4Department of Biological Sciences, The George Washington University, Washington, DC, United States.
Abstract
This data report presents sequence data (16S rRNA gene amplicon sequences, metagenomes, and metagenome-assembled genomes - MAGs) from biofilms and geothermally heated soils (temperatures between 32.6 - 57.6°C) in Fissures 8 and 24 in Hawai'i, USA, 4 years after they were formed by a lava flow during the 2018 Lower Puna eruption. Using DNA extracted from these samples, we generated 587 Mbp and 363 Mbp of raw data from 16S rRNA gene sequencing using universal and archaeal-specific primers, respectively, and 62.7 Gbp of raw data from metagenome sequencing. Using the amplicon sequence data, we produced 276 ASVs from the universal primers and 576 ASVs from the archaeal-specific primers. Using the metagenome data, we reconstructed 82 medium-quality (>50% completeness and <10% contamination) and 61 high-quality (>90% completeness and <5% contamination) prokaryotic MAGs. Fastq files of the raw 16S rRNA gene amplicon sequences and metagenomes, as well as MAG sequences are publicly available on NCBI under BioProject PRJNA1459608. The amplicon sequence data indicated a strong effect of temperature, with communities dominated by Cyanobacteriota, Armatimonadota, and Pseudomonadota. The MAGs were primarily from domain Bacteria, having 142 MAGs, and 1 MAG from Archaea, with most abundance from phyla Chloroflexota, Proteobacteria and Acidobacteriota . These sequencing data provide a valuable resource for researchers interested in the colonization of geothermally heated soils as well as those interested in the microbial diversity and functionality of microorganisms inhabiting geothermally heated soils globally.
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BibTeX
@article{espinosa2026dataset,
title = {{Dataset of amplicons, metagenomes, and metagenome-assembled genomes from geothermal biofilms four years after a volcanic eruption in Hawai'i.}},
author = {Aurelio Espinosa and Christina Pavloudi and Maximillian D Shlafstein and Seager Ray and Pia Sen and Stephen E Noell and Jimmy H Saw},
journal = {Data in brief},
year = {2026},
month = aug,
doi = {10.1016/j.dib.2026.113316},
url = {https://europepmc.org/article/MED/42910101},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Dataset of amplicons, metagenomes, and metagenome-assembled genomes from geothermal biofilms four years after a volcanic eruption in Hawai'i.
AU - Aurelio Espinosa
AU - Christina Pavloudi
AU - Maximillian D Shlafstein
AU - Seager Ray
AU - Pia Sen
AU - Stephen E Noell
AU - Jimmy H Saw
PY - 2026
DA - 2026/08/01
JO - Data in brief
DO - 10.1016/j.dib.2026.113316
UR - https://europepmc.org/article/MED/42910101
AB - This data report presents sequence data (16S rRNA gene amplicon sequences, metagenomes, and metagenome-assembled genomes - MAGs) from biofilms and geothermally heated soils (temperatures between 32.6 - 57.6°C) in Fissures 8 and 24 in Hawai'i, USA, 4 years after they were formed by a lava flow during the 2018 Lower Puna eruption. Using DNA extracted from these samples, we generated 587 Mbp and 363 Mbp of raw data from 16S rRNA gene sequencing using universal and archaeal-specific primers, respectively, and 62.7 Gbp of raw data from metagenome sequencing. Using the amplicon sequence data, we produced 276 ASVs from the universal primers and 576 ASVs from the archaeal-specific primers. Using the metagenome data, we reconstructed 82 medium-quality (>50% completeness and <10% contamination) and 61 high-quality (>90% completeness and <5% contamination) prokaryotic MAGs. Fastq files of the raw 16S rRNA gene amplicon sequences and metagenomes, as well as MAG sequences are publicly available on NCBI under BioProject PRJNA1459608. The amplicon sequence data indicated a strong effect of temperature, with communities dominated by Cyanobacteriota, Armatimonadota, and Pseudomonadota. The MAGs were primarily from domain Bacteria, having 142 MAGs, and 1 MAG from Archaea, with most abundance from phyla Chloroflexota, Proteobacteria and Acidobacteriota . These sequencing data provide a valuable resource for researchers interested in the colonization of geothermally heated soils as well as those interested in the microbial diversity and functionality of microorganisms inhabiting geothermally heated soils globally.
SN - 2352-3409
ER -