Brevican immunoreactivity in the mouse cochlea during development and following noise-induced hearing loss
Abstract
Perineuronal nets (PNNs) regulate synaptic stabilization and neural circuit development in the central nervous system. Although BCAN-associated extracellular matrix specializations have been described in the cochlea, their developmental organization and response to acoustic injury remain poorly understood. Here we characterized BCAN-associated extracellular matrix in the developing and adult mouse cochlea and examined changes following noise-induced hearing loss (NIHL). HAPLN1 and neurocan were broadly detected around inner hair cells from postnatal day 7 (P7), whereas BCAN immunoreactivity was not detected at P7 but was detectable around the inner hair cell (IhC) region at P14. RNA in situ hybridization and exploratory analysis of published single-cell transcriptomic datasets characterized developmental patterns of BCAN mRNA signals in spiral ganglion-associated cell populations. Three-dimensional cochlear clearing and light-sheet imaging qualitatively revealed BCAN immunoreactivity surrounding a subset of cell bodies in the auditory nerve transitional region of adult cochleae. Following permanent-threshold-shift–inducing noise exposure, BCAN immunoreactivity was markedly reduced in the IhC region and appeared less prominent around cell bodies in the auditory nerve transitional region. These findings further characterize BCAN-associated extracellular matrix patterns in the mouse cochlea and describe developmental changes in BCAN immunoreactivity and its reduction following severe NIHL.
Related
- Tissue-resident and recruited mononuclear phagocyte organization in the cochlea under homeostasis and implant-induced inflammation
- Notch1 and Notch2 receptors mediate the prosensory functions of the Notch ligand Jagged1 in the mammalian cochlea
- Comparative proteomics and immunoreactivity of Lonomia venomous caterpillars identify hemolins as predominant toxins
- Epithelial-mesenchymal Wnt crosstalk directs planar cell polarity in the developing cochlea.
- Inner-ear delivery of AAV2-retro robustly labels lateral olivocochlear efferents but results in off-target transfection in the contralateral cochlea and the brain
BibTeX
@article{kim2026brevican,
title = {{Brevican immunoreactivity in the mouse cochlea during development and following noise-induced hearing loss}},
author = {So Young Kim and Nuri Jin and Ho Sun Lee and Min-Hyun Park},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-75639-1},
url = {https://www.nature.com/articles/s41598-026-75639-1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Brevican immunoreactivity in the mouse cochlea during development and following noise-induced hearing loss
AU - So Young Kim
AU - Nuri Jin
AU - Ho Sun Lee
AU - Min-Hyun Park
PY - 2026
DA - 2026/10/13
JO - Scientific Reports
DO - 10.1038/s41598-026-75639-1
UR - https://www.nature.com/articles/s41598-026-75639-1
AB - Perineuronal nets (PNNs) regulate synaptic stabilization and neural circuit development in the central nervous system. Although BCAN-associated extracellular matrix specializations have been described in the cochlea, their developmental organization and response to acoustic injury remain poorly understood. Here we characterized BCAN-associated extracellular matrix in the developing and adult mouse cochlea and examined changes following noise-induced hearing loss (NIHL). HAPLN1 and neurocan were broadly detected around inner hair cells from postnatal day 7 (P7), whereas BCAN immunoreactivity was not detected at P7 but was detectable around the inner hair cell (IhC) region at P14. RNA in situ hybridization and exploratory analysis of published single-cell transcriptomic datasets characterized developmental patterns of BCAN mRNA signals in spiral ganglion-associated cell populations. Three-dimensional cochlear clearing and light-sheet imaging qualitatively revealed BCAN immunoreactivity surrounding a subset of cell bodies in the auditory nerve transitional region of adult cochleae. Following permanent-threshold-shift–inducing noise exposure, BCAN immunoreactivity was markedly reduced in the IhC region and appeared less prominent around cell bodies in the auditory nerve transitional region. These findings further characterize BCAN-associated extracellular matrix patterns in the mouse cochlea and describe developmental changes in BCAN immunoreactivity and its reduction following severe NIHL.
SN - 2045-2322
ER -