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Peer-reviewedCC BYBiophysics reports

Screening for putative essential genes in Brucella abortus strains A19 and S2308 using transposon mutagenesis.

  • Siwei Guo1
  • Weidong Bu2
  • Fangfang Guo2
  • Dianyu Li1
  • Liyuan Liu1
  • Xiaxia Liu1
  • Yuefeng Chu1
  • Jian Xu1
  1. 1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
  2. 2Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
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Abstract

Brucella is a zoonotic pathogen that causes substantial economic losses in the livestock industry. However, the categorization of functional genes in Brucella requires further refinement. This knowledge gap was addressed by classifying gene essentiality through constructing a transposon mutant library. Mariner transposon mutant libraries were independently constructed for Brucella abortus A19 and S2308. Transposon sequencing was used to visualize the insertion sites within the mutant library, which revealed a uniform transposon distribution in both genomes and high library saturation. Furthermore, 451 and 393 putative essential genes were identified for B. abortus A19 and S2308, respectively. The functional enrichment analysis of these genes revealed that cofactor biosynthesis was the most highly represented, followed by ribosomal biogenesis, carbon metabolism, amino acid biosynthesis, and oxidative phosphorylation. Many of these genes encode known antimicrobial drug targets. This study provides a foundational resource for Brucella research, and the mutant library serves as a tool for screening functional Brucella genes.

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Cite

BibTeXRIS
BibTeX
@article{guo2026screening,
  title = {{Screening for putative essential genes in Brucella abortus strains A19 and S2308 using transposon mutagenesis.}},
  author = {Siwei Guo and Weidong Bu and Fangfang Guo and Dianyu Li and Liyuan Liu and Xiaxia Liu and Yuefeng Chu and Jian Xu},
  journal = {Biophysics reports},
  year = {2026},
  month = oct,
  doi = {10.52601/bpr.2025.250050},
  url = {https://europepmc.org/article/MED/42997144},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Screening for putative essential genes in Brucella abortus strains A19 and S2308 using transposon mutagenesis.
AU  - Siwei Guo
AU  - Weidong Bu
AU  - Fangfang Guo
AU  - Dianyu Li
AU  - Liyuan Liu
AU  - Xiaxia Liu
AU  - Yuefeng Chu
AU  - Jian Xu
PY  - 2026
DA  - 2026/10/31
JO  - Biophysics reports
DO  - 10.52601/bpr.2025.250050
UR  - https://europepmc.org/article/MED/42997144
AB  - Brucella is a zoonotic pathogen that causes substantial economic losses in the livestock industry. However, the categorization of functional genes in Brucella requires further refinement. This knowledge gap was addressed by classifying gene essentiality through constructing a transposon mutant library. Mariner transposon mutant libraries were independently constructed for Brucella abortus A19 and S2308. Transposon sequencing was used to visualize the insertion sites within the mutant library, which revealed a uniform transposon distribution in both genomes and high library saturation. Furthermore, 451 and 393 putative essential genes were identified for B. abortus A19 and S2308, respectively. The functional enrichment analysis of these genes revealed that cofactor biosynthesis was the most highly represented, followed by ribosomal biogenesis, carbon metabolism, amino acid biosynthesis, and oxidative phosphorylation. Many of these genes encode known antimicrobial drug targets. This study provides a foundational resource for Brucella research, and the mutant library serves as a tool for screening functional Brucella genes.
SN  - 2364-3439
SN  - 2364-3420
ER  -