Identification of a novel solid stem QTL in hexaploid wheat contributing resistance to wheat stem sawfly
- 1Plant Sciences and Plant Pathology, College of Agriculture, Montana State University , Bozeman, MT 59717 ,
- 2Cereals Crops Research Improvement Unit, United States Department of Agriculture (USDA) Agricultural Research Service (ARS) , Fargo, ND 58102 ,
Abstract
Stem solidness is a heritable quantitative trait that provides resistance against wheat stem sawfly (WSS) infestation in durum and hexaploid wheat. Stem solidness arises from the action of multiple genetic regions contributing to temporal stem pith production, identified in ancient landraces and cultivars of wheat. However, uniform expression of stem pith across all internodes and throughout wheat's life cycle remain unobserved. In the present study, we dissected the genetic architecture of the solid stem trait by leveraging a cultivar from the International Maize and Wheat Improvement Center, ‘CIMMYT-9263’. A recombinant inbred mapping population comprising 146 lines was created by crossing ‘CIMMYT-9263’ with a Montana-adapted, photoperiod-insensitive, WSS susceptible variety, ‘Hi-Line’. Multiyear phenotypic assessment of the solid stem trait at stem elongation (Zadoks 32) and maturity (Zadoks 77), along with WSS field stem cutting (%), revealed QTL regions contributing to host plant resistance. We report a major QTL region on the long arm of chromosome 3A spanning the physical distance 730,477,755 to 739,555,941 bp, named, Qss.msub.3AL-2. The allele Qss.msub.3AL-2.b contributed from the ‘CIMMYT-9263’ controls for increased stem pith production and reduced stem cutting. Markers associated with the Qss.msub.3AL-2.b solid stem allele were used to create Kompetitive Allele Specific Primers (KASP) for marker assisted selection (MAS). A small effect QTL on chromosome 3D was also identified controlling for temporal stem solidness. The two solid stem QTL identified in the present study will contribute to creating cultivars carrying multiple solid stem alleles for improving WSS resistance.
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BibTeX
@article{isha2026identification,
title = {{Identification of a novel solid stem QTL in hexaploid wheat contributing resistance to wheat stem sawfly}},
author = {Ishita Isha and Jason Fiedler and Raja Sekhar Nandety and Jennifer Lachowiec and Jessica Kansman and Nancy Blake and Hwa Young Heo and Lucas A Wright and Jason P Cook},
journal = {G3: Genes, Genomes, Genetics},
year = {2026},
month = oct,
doi = {10.1093/g3journal/jkag282},
url = {https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag282/8860175},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Identification of a novel solid stem QTL in hexaploid wheat contributing resistance to wheat stem sawfly
AU - Ishita Isha
AU - Jason Fiedler
AU - Raja Sekhar Nandety
AU - Jennifer Lachowiec
AU - Jessica Kansman
AU - Nancy Blake
AU - Hwa Young Heo
AU - Lucas A Wright
AU - Jason P Cook
PY - 2026
DA - 2026/10/03
JO - G3: Genes, Genomes, Genetics
DO - 10.1093/g3journal/jkag282
UR - https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag282/8860175
AB - Stem solidness is a heritable quantitative trait that provides resistance against wheat stem sawfly (WSS) infestation in durum and hexaploid wheat. Stem solidness arises from the action of multiple genetic regions contributing to temporal stem pith production, identified in ancient landraces and cultivars of wheat. However, uniform expression of stem pith across all internodes and throughout wheat's life cycle remain unobserved. In the present study, we dissected the genetic architecture of the solid stem trait by leveraging a cultivar from the International Maize and Wheat Improvement Center, ‘CIMMYT-9263’. A recombinant inbred mapping population comprising 146 lines was created by crossing ‘CIMMYT-9263’ with a Montana-adapted, photoperiod-insensitive, WSS susceptible variety, ‘Hi-Line’. Multiyear phenotypic assessment of the solid stem trait at stem elongation (Zadoks 32) and maturity (Zadoks 77), along with WSS field stem cutting (%), revealed QTL regions contributing to host plant resistance. We report a major QTL region on the long arm of chromosome 3A spanning the physical distance 730,477,755 to 739,555,941 bp, named, Qss.msub.3AL-2. The allele Qss.msub.3AL-2.b contributed from the ‘CIMMYT-9263’ controls for increased stem pith production and reduced stem cutting. Markers associated with the Qss.msub.3AL-2.b solid stem allele were used to create Kompetitive Allele Specific Primers (KASP) for marker assisted selection (MAS). A small effect QTL on chromosome 3D was also identified controlling for temporal stem solidness. The two solid stem QTL identified in the present study will contribute to creating cultivars carrying multiple solid stem alleles for improving WSS resistance.
SN - 2160-1836
ER -