Advancing Neurological Liquid Biopsy via Six-Base Sequencing of Cerebrospinal Fluid Cell-Free DNA

Abstract

Real-time molecular insights into the human brain are crucial for understanding neurological conditions and development of soluble biomarkers but challenged by the limited access to target tissue. Here we utilize 6-base sequencing to profile DNA modifications of cell-free DNA released from neural cells into cerebrospinal fluid. This strategy provides molecular, quantitative and qualitative insights into the ongoing neurodegenerative biology, which paves the way for liquid biopsy approaches to study neurological conditions.

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BibTeX RIS

BibTeX

@misc{cucuzza2026advancing,
  title = {{Advancing Neurological Liquid Biopsy via Six-Base Sequencing of Cerebrospinal Fluid Cell-Free DNA}},
  author = {C. Starvaggi Cucuzza and C. Rao Prakash and C. Nolan and M. H. Sepehri and A. Simeone and L. Sarre and T. Charlesworth and F. Puddu and Y. Han and M. Khademi and V. Danylaite Karrenbauer and B. Evertsson and K. Fink and P. Svenningsson and C. Ingre and C. Seitz and F. Fang and R. Constantinescu and L. Novakova and E. P. Thelin and F. Piehl and L. Kular and M. Needhamsen and M. Jagodic},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.06.749692},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.06.749692v1},
  note = {CC BY-NC-ND},
}

RIS

TY  - UNPB
TI  - Advancing Neurological Liquid Biopsy via Six-Base Sequencing of Cerebrospinal Fluid Cell-Free DNA
AU  - C. Starvaggi Cucuzza
AU  - C. Rao Prakash
AU  - C. Nolan
AU  - M. H. Sepehri
AU  - A. Simeone
AU  - L. Sarre
AU  - T. Charlesworth
AU  - F. Puddu
AU  - Y. Han
AU  - M. Khademi
AU  - V. Danylaite Karrenbauer
AU  - B. Evertsson
AU  - K. Fink
AU  - P. Svenningsson
AU  - C. Ingre
AU  - C. Seitz
AU  - F. Fang
AU  - R. Constantinescu
AU  - L. Novakova
AU  - E. P. Thelin
AU  - F. Piehl
AU  - L. Kular
AU  - M. Needhamsen
AU  - M. Jagodic
PY  - 2026
DA  - 2026/09/06
PB  - bioRxiv
DO  - 10.64898/2026.09.06.749692
UR  - https://www.biorxiv.org/content/10.64898/2026.09.06.749692v1
AB  - Real-time molecular insights into the human brain are crucial for understanding neurological conditions and development of soluble biomarkers but challenged by the limited access to target tissue. Here we utilize 6-base sequencing to profile DNA modifications of cell-free DNA released from neural cells into cerebrospinal fluid. This strategy provides molecular, quantitative and qualitative insights into the ongoing neurodegenerative biology, which paves the way for liquid biopsy approaches to study neurological conditions.
ER  -

Abstract

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